Volume 111, Issue 6, Pages (September 2016)

Slides:

Advertisements

Similar presentations

How Do Thermophilic Proteins and Proteomes Withstand High Temperature? Lucas Sawle, Kingshuk Ghosh Biophysical Journal Volume 101, Issue 1, Pages

Advertisements

On the Thermodynamic Efficiency of Ca2+-ATPase Molecular Machines Anders Lervik, Fernando Bresme, Signe Kjelstrup, J. Miguel Rubí Biophysical Journal Volume.

Analysis of Kinetic Intermediates in Single-Particle Dwell-Time Distributions Daniel L. Floyd, Stephen C. Harrison, Antoine M. van Oijen Biophysical Journal.

Counter-Intuitive Stochastic Behavior of Simple Gene Circuits with Negative Feedback Tatiana T. Marquez-Lago, Jörg Stelling Biophysical Journal Volume.

Structure and Dynamics of ER: Minimal Networks and Biophysical Constraints Congping Lin, Yiwei Zhang, Imogen Sparkes, Peter Ashwin Biophysical Journal.

Calibration of Optical Tweezers for In Vivo Force Measurements: How do Different Approaches Compare? Yonggun Jun, Suvranta K. Tripathy, Babu R.J. Narayanareddy,

Elucidating the Locking Mechanism of Peptides onto Growing Amyloid Fibrils through Transition Path Sampling Marieke Schor, Jocelyne Vreede, Peter G. Bolhuis.

A Multiscale Red Blood Cell Model with Accurate Mechanics, Rheology, and Dynamics Dmitry A. Fedosov, Bruce Caswell, George Em Karniadakis Biophysical Journal.

X-Ray Absorption Spectroscopy of Dinuclear Metallohydrolases David L. Tierney, Gerhard Schenk Biophysical Journal Volume 107, Issue 6, Pages

Cytoskeletal Polymer Networks: Viscoelastic Properties are Determined by the Microscopic Interaction Potential of Cross-links O. Lieleg, K.M. Schmoller,

Photochemical Reaction Dynamics of the Primary Event of Vision Studied by Means of a Hybrid Molecular Simulation Shigehiko Hayashi, Emad Tajkhorshid, Klaus.

Lever-Arm Mechanics of Processive Myosins Yujie Sun, Yale E. Goldman Biophysical Journal Volume 101, Issue 1, Pages 1-11 (July 2011) DOI: /j.bpj

The Importance of the Hook Region of the Cochlea for Bone-Conduction Hearing Namkeun Kim, Charles R. Steele, Sunil Puria Biophysical Journal Volume 107,

The Interrelations among Stochastic Pacing, Stability, and Memory in the Heart Hila Dvir, Sharon Zlochiver Biophysical Journal Volume 107, Issue 4, Pages.

Cell Traction Forces Direct Fibronectin Matrix Assembly Christopher A. Lemmon, Christopher S. Chen, Lewis H. Romer Biophysical Journal Volume 96, Issue.

Quantifying the Rheological and Hemodynamic Characteristics of Sickle Cell Anemia Huan Lei, George Em Karniadakis Biophysical Journal Volume 102, Issue.

Nonlinear Poisson Equation for Heterogeneous Media Langhua Hu, Guo-Wei Wei Biophysical Journal Volume 103, Issue 4, Pages (August 2012) DOI: /j.bpj

Direct Measurements of the Mechanical Stability of Zinc-Thiolate Bonds in Rubredoxin by Single-Molecule Atomic Force Microscopy Peng Zheng, Hongbin Li.

Maryam Sayadi, Seiichiro Tanizaki, Michael Feig Biophysical Journal

Volume 109, Issue 6, Pages (September 2015)

Volume 112, Issue 10, Pages (May 2017)

Volume 111, Issue 7, Pages (October 2016)

Volume 107, Issue 2, Pages (July 2014)

Volume 100, Issue 3, (February 2011)

A Dynamics Criterion to Determine Allostery

Volume 103, Issue 5, Pages (September 2012)

Probing Membrane Order and Topography in Supported Lipid Bilayers by Combined Polarized Total Internal Reflection Fluorescence-Atomic Force Microscopy

Volume 104, Issue 1, Pages (January 2013)

Benoit Tesson, Michael I. Latz Biophysical Journal

Guang-Kui Xu, Xi-Qiao Feng, Huajian Gao Biophysical Journal

SAXS versus FRET: A Matter of Heterogeneity?

Volume 98, Issue 12, Pages (June 2010)

Volume 93, Issue 6, Pages (September 2007)

Local Viscoelastic Properties of Live Cells Investigated Using Dynamic and Quasi-Static Atomic Force Microscopy Methods Alexander Cartagena, Arvind Raman

Nanoscale Measurement of the Dielectric Constant of Supported Lipid Bilayers in Aqueous Solutions with Electrostatic Force Microscopy G. Gramse, A. Dols-Perez,

Susanne Karsch, Deqing Kong, Jörg Großhans, Andreas Janshoff

Maxim E. Dokukin, Nataliia V. Guz, Igor Sokolov Biophysical Journal

Huiyan Wu, Guangyi Zhao, Hongfei Zu, James H.-C. Wang, Qing-Ming Wang

Nanopore Sequencing: Forcing Improved Resolution

Volume 111, Issue 10, Pages (November 2016)

Volume 114, Issue 1, Pages (January 2018)

Recognition Imaging of Acetylated Chromatin Using a DNA Aptamer

Stiffness Tomography by Atomic Force Microscopy

Volume 110, Issue 11, Pages (June 2016)

Unlocking Collagen Proteolysis with a Gentle Pull

Volume 112, Issue 2, Pages (January 2017)

Volume 91, Issue 9, Pages (November 2006)

Dmitry A. Fedosov, Bruce Caswell, George Em Karniadakis

Volume 75, Issue 2, Pages (August 1998)

Volume 111, Issue 2, Pages (July 2016)

Viscoelasticity as a Biomarker for High-Throughput Flow Cytometry

Quantitative Image Restoration in Bright Field Optical Microscopy

Volume 111, Issue 6, Pages (September 2016)

Yiider Tseng, Thomas P. Kole, Denis Wirtz Biophysical Journal

Ralf Jansen, Wojciech Dzwolak, Roland Winter Biophysical Journal

Dynamics of Active Semiflexible Polymers

Kinetics of Surface-Driven Self-Assembly and Fatigue-Induced Disassembly of a Virus- Based Nanocoating Alejandro Valbuena, Mauricio G. Mateu Biophysical.

Volume 99, Issue 6, Pages (September 2010)

Volume 97, Issue 8, Pages (October 2009)

Volume 105, Issue 10, Pages (November 2013)

Huan-Xiang Zhou, Osman Bilsel Biophysical Journal

Nevra Ozer, Celia A. Schiffer, Turkan Haliloglu Biophysical Journal

Volume 101, Issue 11, Pages (December 2011)

Nanoscale Measurement of the Dielectric Constant of Supported Lipid Bilayers in Aqueous Solutions with Electrostatic Force Microscopy G. Gramse, A. Dols-Perez,

Volume 113, Issue 3, Pages (August 2017)

Drug-Induced Changes of Cytoskeletal Structure and Mechanics in Fibroblasts: An Atomic Force Microscopy Study Christian Rotsch, Manfred Radmacher Biophysical.

Systems Biophysics: Multiscale Biophysical Modeling of Organ Systems

Main Phase Transitions in Supported Lipid Single-Bilayer

Prediction of Cell Alignment on Cyclically Strained Grooved Substrates

Dynamics of Snake-like Swarming Behavior of Vibrio alginolyticus

Presentation transcript:

Volume 111, Issue 6, Pages 1173-1179 (September 2016) Identification of HIV-1–Based Virus-like Particles by Multifrequency Atomic Force Microscopy Irene González-Domínguez, Sonia Gutiérrez-Granados, Laura Cervera, Francesc Gòdia, Neus Domingo Biophysical Journal Volume 111, Issue 6, Pages 1173-1179 (September 2016) DOI: 10.1016/j.bpj.2016.07.046 Copyright © 2016 Biophysical Society Terms and Conditions

Figure 1 EM analysis of HIV-1 Gag VLP preparations. (a) TEM analysis. (b) SEM analysis, from deposits on carbon-coated grids. (Straight blue arrows) HIV-1 Gag VLPs; (red dashed arrows) cellular vesicle structures. To see this figure in color, go online. Biophysical Journal 2016 111, 1173-1179DOI: (10.1016/j.bpj.2016.07.046) Copyright © 2016 Biophysical Society Terms and Conditions

Figure 2 TEM and AFM imaging of an individual HIV-1 Gag VLP. (a) TEM image. (b) AFM 3D topography imaging. (c) AFM amplitude and (d) phase image of the fundamental mode. (e) Second amplitude (A2) and (f) phase (Φ2) images of bimodal AFM, corresponding to the dynamics of the first excited eigenmode. (g) First excited eigenmode frequency shift (Δf2) image of AM-FM viscoelastic mapping mode, and the corresponding calculated (h) Young’s modulus image. To see this figure in color, go online. Biophysical Journal 2016 111, 1173-1179DOI: (10.1016/j.bpj.2016.07.046) Copyright © 2016 Biophysical Society Terms and Conditions

Figure 3 Individual VLP deposited on mica substrate. (a) AFM topography image of VLPs on mica substrate. (b) 3D topography image of a single VLP structure. (c) Δf2 imaging of an isolated VLP structure, as obtained by AM-FM viscoelastic mapping mode and (d) the corresponding calculated Young’s modulus distribution. To see this figure in color, go online. Biophysical Journal 2016 111, 1173-1179DOI: (10.1016/j.bpj.2016.07.046) Copyright © 2016 Biophysical Society Terms and Conditions

Figure 4 “Mock” sample deposited on mica substrate. (a) AFM topography image of empty cellular vesicles deposited on mica substrate; (b) corresponding AFM phase image, showing the contrast due to the presence of vesicles. To see this figure in color, go online. Biophysical Journal 2016 111, 1173-1179DOI: (10.1016/j.bpj.2016.07.046) Copyright © 2016 Biophysical Society Terms and Conditions