Koji Matsuura, Ph. D. , Takuya Uozumi, M. S. , Takuya Furuichi, Ph. D

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Presentation transcript:

A microfluidic device to reduce treatment time of intracytoplasmic sperm injection Koji Matsuura, Ph.D., Takuya Uozumi, M.S., Takuya Furuichi, Ph.D., Ikuyo Sugimoto, M.S., Mieko Kodama, B.S., Hiroaki Funahashi, Ph.D. Fertility and Sterility Volume 99, Issue 2, Pages 400-407 (February 2013) DOI: 10.1016/j.fertnstert.2012.10.022 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 The design of the microfluidic device. (A) Schematic view of the device. (B) Image of the device. Asterisks represent the inlet and outlet of the device. The letters a, b, and c represent the injection channels, collection chamber, and narrow channels, respectively. (C) The schematic diagram of the protocol for using this device. (1) Medium is injected from the inlet to fill the microfluidic channel. (2) Diluted semen is injected into the channel. (3) Top thin resin layer on the collection chamber is pricked by a glass needle or pipette. (4) Sperm is sucked into a micropipette and transferred to a microdrop. Fertility and Sterility 2013 99, 400-407DOI: (10.1016/j.fertnstert.2012.10.022) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 The schematic diagram to compare ICSI treatments using microdroplets and the microfluidic device. The treatment time includes selection of sperm and injection of sperm into oocyte. Fertility and Sterility 2013 99, 400-407DOI: (10.1016/j.fertnstert.2012.10.022) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 (A) ICSI examination: comparison of sperm injection treatment time. Light and dark gray bars are the average treatment times of the conventional microdroplet method and the method using the microfluidic device, respectively. These data are presented as mean ± SEM. A statistically significant difference is indicated: *P<.05. (B) Comparison of sperm concentration obtained with and without the use of microfluidic device. Light and dark gray bars are the average sperm concentrations of the prepared semen and the method using the microfluidic device, respectively. These data are presented as mean ± SEM. A statistically significant difference is indicated: *P<.05. Fertility and Sterility 2013 99, 400-407DOI: (10.1016/j.fertnstert.2012.10.022) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 4 (A) Schematic view of the device to explain the observation area of microspheres using fluorescence microscope. (B) and (C) Recorded images at the top and the bottom of the junction of the chamber and the channel of the microfluidic device. White particles in the binarized images show the fluorescent microspheres. (D) and (E) Representative time-resolved confocal fluorescent images of the microspheres with fluid flow. Arrows show direction of the particle motion. Fertility and Sterility 2013 99, 400-407DOI: (10.1016/j.fertnstert.2012.10.022) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions