Volume 103, Issue 11, Pages (December 2012)

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Presentation transcript:

Volume 103, Issue 11, Pages 2399-2407 (December 2012) Fibrin Clot Structure and Mechanics Associated with Specific Oxidation of Methionine Residues in Fibrinogen Katie M. Weigandt, Nathan White, Dominic Chung, Erica Ellingson, Yi Wang, Xiaoyun Fu, Danilo C. Pozzo Biophysical Journal Volume 103, Issue 11, Pages 2399-2407 (December 2012) DOI: 10.1016/j.bpj.2012.10.036 Copyright © 2012 Biophysical Society Terms and Conditions

Figure 1 Fibrinogen molecule with all methionine residues circled and highly oxidized methionine residues labeled. The crystal structure was rendered from the Protein Data Bank (23,24). The α-C domain was added schematically with dotted lines, including the ordered N- and C-terminal subdomains (25). Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions

Figure 2 Absorbance at 350 nm for 1 mg/mL fibrin gels plotted as a function of time during gelation. Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions

Figure 3 SEM images of fibrin gels from HOCl oxidized fibrinogen at 1000× (top) and 20,000× (bottom) magnification. Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions

Figure 4 Elastic (G′) and viscous (G″) moduli of oxidized and baseline fibrin gels plotted as a function of time during gelation. Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions

Figure 5 Linear elastic modulus of the fibrin gels plotted against the fibrinogen concentration. Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions

Figure 6 Instantaneous modulus (GInst) of fibrin gels with various concentrations of fibrinogen and oxidized to different levels with 0, 50, and 150 μmol HOCl/g fibrinogen. The data plotted are the average of at least three trials, with error bars corresponding to the standard deviation. Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions

Figure 7 Fibrinolysis induced by tPA and plasminogen in 2.2 mg/mL fibrin gels formed from fibrinogen treated with 0, 50, and 150 μmol/g fibrinogen as monitored by small-amplitude oscillatory rheology. The lysis time for these specific gels is marked with an arrow. The reported lysis times represent the average of three trials. Biophysical Journal 2012 103, 2399-2407DOI: (10.1016/j.bpj.2012.10.036) Copyright © 2012 Biophysical Society Terms and Conditions