Reduced adenylyl cyclase activation with no decrease in β-adrenergic receptors in basenji greyhound leukocytes: Relevance to β-adrenergic responses in.

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Reduced adenylyl cyclase activation with no decrease in β-adrenergic receptors in basenji greyhound leukocytes: Relevance to β-adrenergic responses in airway smooth muscle Charles W. Emala, MD, Michael A. Levine, MD, Arash Aryana, MS, Joseph B. Margolick, MD, PhD, Carol A. Hirshman, MD Journal of Allergy and Clinical Immunology Volume 95, Issue 4, Pages 860-867 (April 1995) DOI: 10.1016/S0091-6749(95)70130-3 Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 1 Representative saturation binding isotherm with Scatchard transformation in MNL membranes from BG and mongrel dogs. Membranes were incubated with 125I-cyanopindolol (0 to 400 pmol/L) in the presence or absence of (-)-propranolol (10 μmol/L). Data are representative of experiments performed in MNL membranes of six dogs in each group. ICYP, 125I-cyanopindolol. Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Adenylyl cyclase activities in MNL membranes from BG and mongrel dogs. A, Adenylyl cyclase activity in response to isoproterenol (100 μmol/L) and PGE1 (10 μmol/L) expressed as the percent increase in activity over GTP (10 μmol/L) alone. B, Adenylyl cyclase activity in MNL membranes of BG and mongrel dogs in response to forskolin (10 μmol/L) and NaF (10 mmol/L) expressed as a percent increase over basal (unstimulated) activity. Data are expressed and means ± SEM (n = 9 for mongrel group and n = 13 for BG group). Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 Adenylyl cyclase activities in MNL membranes from BG and mongrel dogs. A, Adenylyl cyclase activity in response to isoproterenol (100 μmol/L) and PGE1 (10 μmol/L) expressed as the percent increase in activity over GTP (10 μmol/L) alone. B, Adenylyl cyclase activity in MNL membranes of BG and mongrel dogs in response to forskolin (10 μmol/L) and NaF (10 mmol/L) expressed as a percent increase over basal (unstimulated) activity. Data are expressed and means ± SEM (n = 9 for mongrel group and n = 13 for BG group). Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 3 Adenylyl cyclase activities in MNL membranes from BG and mongrel dogs. Adenylyl cyclase activity in response to isoproterenol is expressed as percent of increase in activity over GTP alone. Data are expressed as means ± SEM (n = 4) and were analyzed by two-way analysis of variance with a Bonferroni correction for pairwise comparisons. The dose response to isoproterenol was significantly decreased in membranes of BG MNLs as compared with mongrel MNLs (p = 0.0005). Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 4 Lymphocyte subsets in peripheral blood of BG and mongrel dogs. Proportions of lymphocytes were determined by flow cytometry (see Methods section) and are expressed as percent of mononuclear cells. Data are expressed as means ± SEM (n = 6). Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 5 Densitometric quantification of Gsα protein content in MNL membranes of BG and mongrel dogs. A, Band intensity is expressed as percent maximum within each immunoblot. B, A representative immunoblot of Gsα from BG and mongrel membranes. Bands at 45 kd and 52 kd were visible in MNL membranes from BG and mongrel dogs. Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 5 Densitometric quantification of Gsα protein content in MNL membranes of BG and mongrel dogs. A, Band intensity is expressed as percent maximum within each immunoblot. B, A representative immunoblot of Gsα from BG and mongrel membranes. Bands at 45 kd and 52 kd were visible in MNL membranes from BG and mongrel dogs. Journal of Allergy and Clinical Immunology 1995 95, 860-867DOI: (10.1016/S0091-6749(95)70130-3) Copyright © 1995 Mosby, Inc. Terms and Conditions