Mechanosensitive transient receptor potential vanilloid type 1 channels contribute to vascular remodeling of rat fistula veins Yih-Sharng Chen, MD, PhD,

Slides:

Advertisements

Similar presentations

Vascular changes at the puncture segments of arteriovenous fistula for hemodialysis access Ju-Feng Hsiao, MD, Hsin-Hua Chou, MD, Lung-An Hsu, MD, PhD,

Advertisements

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis April E. Nedeau, MD,

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats Zhenhai Yu, MD, Keisuke.

George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S

Combination of ADMSCs and chondrocytes reduces hypertrophy and improves the functional properties of osteoarthritic cartilage M.R. Ahmed, A. Mehmood,

Volume 85, Issue 2, Pages (January 2014)

Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,

Chun-Yu Wong, MD, Margreet R. de Vries, BSc, Yang Wang, MD, Joost R

VCAM-1 siRNA reduces neointimal formation after surgical mechanical injury of the rat carotid artery Yanming Qu, MD, Xiangen Shi, MD, Hongwei Zhang,

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9 Ada W.Y. Chung, PhD, Pooja.

Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells Paul DiMuzio, MD, Thomas Tulenko, PhD Journal.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model Jianping Dai, MD, PhD, Liliane.

Jeffrey E. Hull, MD, Boris V. Balakin, PhD, Brad M

Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps Francesca Polito, PhD, Alessandra Bitto, MD, PhD, Mariarosaria.

In vivo assessment of endothelial function in human lower extremity arteries Vikram S. Kashyap, MD, Ryan O. Lakin, MD, Lindsay E. Feiten, BS, Paul D.

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Increased connexin43 expression in human saphenous veins in culture is associated with intimal hyperplasia Sébastien Déglise, MD, David Martin, MS, Hervé.

Peritherapeutic quantitative flow analysis of arteriovenous malformation on digital subtraction angiography Tzung-Chi Huang, PhD, Tung-Hsin Wu, PhD,

Journal of Vascular Surgery

Differential expression of elastin assembly genes in patients with Stanford Type A aortic dissection using microarray analysis Bernice L.Y. Cheuk, PhD,

Metformin stimulates ischemia-induced revascularization through an eNOS dependent pathway in the ischemic hindlimb mice model Noriko Takahashi, MD, Rei.

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Pioglitazone preserves vein graft integrity in a rat aortic interposition model Zhi Chen, MD, Tomomi Hasegawa, MD, PhD, Akiko Tanaka, MD, Yutaka Okita,

The effect of matrix metalloproteinase 2 and matrix metalloproteinase 2/9 deletion in experimental post-thrombotic vein wall remodeling Kristopher B.

Daniel J. Wong, MD, Daniel Y. Lu, MD, Clinton D

Overexpression of transforming growth factor–β1 correlates with increased synthesis of nitric oxide synthase in varicose veins Theresa Jacob, PhD, Anil.

Tissue factor pathway inhibitor-2 is induced by fluid shear stress in vascular smooth muscle cells and affects cell proliferation and survival Johan.

Endothelial nitric oxide synthase affects both early and late collateral arterial adaptation and blood flow recovery after induction of hind limb ischemia.

Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,

Simvastatin suppresses experimental aortic aneurysm expansion

Therapeutic site selection is important for the successful development of collateral vessels Ayako Nishiyama, MD, PhD, Hiroyuki Koyama, MD, PhD, Tetsuro.

Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,

Tumescent anesthesia reduces pain associated with balloon angioplasty of hemodialysis fistulas Wen-Yu V. Haines, MD, Ryan Deets, BS, Ning Lu, BS, John.

Luigi Pascarella, MD, Geert W. Schmid-Schönbein, PhD, John Bergan, MD

Adventitial endothelial implants reduce matrix metalloproteinase-2 expression and increase luminal diameter in porcine arteriovenous grafts Helen M.

Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins Xavier Berard, MD, PhD, Sébastien Déglise, MD, Florian Alonso, PhD,

A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure Akiko.

Autophagy-mediated stress response in motor neurons after hypothermic spinal cord ischemia in rabbits Satoshi Fujita, MD, Masahiro Sakurai, MD, PhD,

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Prolonged increases in vein wall tension increase matrix metalloproteinases and decrease constriction in rat vena cava: Potential implications in varicose.

Extracorporeal shock wave therapy ameliorates secondary lymphedema by promoting lymphangiogenesis Masayuki Kubo, PhD, Tao-Sheng Li, MD, PhD, Takahiro.

Characterization of primary and restenotic atherosclerotic plaque from the superficial femoral artery: Potential role of Smad3 in regulation of SMC proliferation

Gene expression changes evoked in a venous segment exposed to arterial flow Deborah Abeles, MD, Stephanie Kwei, MD, George Stavrakis, MS, Yuzhi Zhang,

The effects of ginsenoside Rb1 on endothelial damage and ghrelin expression induced by hyperhomocysteine Zhiwei Xu, PhD, Taohua Lan, MD, Weikang Wu,

The molecular mechanisms of hemodialysis vascular access failure

Michael D. Sgroi, MD, Madhukar S. Patel, MD, Samuel E

Nitric oxide inhibition increases matrix metalloproteinase–9 expression by rat aortic smooth muscle cells in vitro Gilbert R. Upchurch, MD, John W. Ford,

The role of short-term oxygen administration in the prevention of intimal hyperplasia Charu Lata, MBBS, Derrick Green, MD, Jing Wan, MD, Sabita Roy, PhD,

Vascular complications of cardiac catheterization

Role of matrix metalloproteinases 1, 2, and 9 and tissue inhibitor of matrix metalloproteinase-1 in chronic venous insufficiency Satoshi Saito, MD, Matthew.

Traumatic arteriovenous fistula 52 years after injury

Pharmacologic relaxation of vein grafts is beneficial compared with pressure distention caused by upregulation of endothelial nitric oxide synthase and.

In vitro differences between smooth muscle cells derived from varicose veins and normal veins Ying Xiao, PhD, Zhibin Huang, MD, Henghui Yin, PhD, Ying.

Autophagy-mediated stress response in motor neuron after transient ischemia in rabbits Hironori Baba, MD, Masahiro Sakurai, MD, PhD, Koji Abe, MD, PhD,

Reply Journal of Vascular Surgery

Metalloproteinase expression in venous aneurysms

Deep vein thrombosis resolution is impaired in diet-induced type 2 diabetic mice Fatiha Bouzeghrane, PhD, Xiaochun Zhang, MD, BSc, Guylaine Gevry, BSc,

Prevention of vascular access hand ischemia using the axillary artery as inflow William Jennings, MD, Robert Brown, MD, John Blebea, MD, MBA, Kevin Taubman,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Effect of Vein Graft Harvesting on Endothelial Nitric Oxide Synthase and Nitric Oxide Production Michael R. Dashwood, PhD, Kay Savage, PhD, Audrey Dooley,

Controlled release of small interfering RNA targeting midkine attenuates intimal hyperplasia in vein grafts Hiroshi Banno, MD, Yoshifumi Takei, PhD,

Activation of transglutaminase type 2 for aortic wall protection in a rat abdominal aortic aneurysm formation Takashi Munezane, MD, Tomomi Hasegawa,

Differential transcriptional activation of matrix metalloproteinase-2 and membrane type-1 matrix metalloproteinase by experimental deep venous thrombosis.

Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats Chih-Yang Chan, MD, Yih-Sharng Chen, MD,

A novel rat model of abdominal aortic aneurysm using a combination of intraluminal elastase infusion and extraluminal calcium chloride exposure Akiko.

Gene expression changes evoked in a venous segment exposed to arterial flow Deborah Abeles, MD, Stephanie Kwei, MD, George Stavrakis, MS, Yuzhi Zhang,

Presentation transcript:

Mechanosensitive transient receptor potential vanilloid type 1 channels contribute to vascular remodeling of rat fistula veins Yih-Sharng Chen, MD, PhD, Ming-Jen Lu, MD, Ho-Shiang Huang, MD, PhD, Ming-Chieh Ma, PhD Journal of Vascular Surgery Volume 52, Issue 5, Pages 1310-1320 (November 2010) DOI: 10.1016/j.jvs.2010.05.095 Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 1 Hemodynamic changes and venous remodeling in rat arteriovenous fistula (AVF). A, The anastomosis of the femoral artery and vein was created by invagination of the cut end of the artery (b) into the vein (a). B, Changes in blood flow, wall shear stress (WSS), intravascular pressure, the internal radius, and width in control and fistula veins at various time points. C, Representative images show marked vasodilatation of the iliac vein at the fistula site (indicated by double arrows in yellow) compared with the contralateral site on day 14 and 28. D, Changes in the thickness of intima (upper) and media (middle), and the ratio of intima to media (IM) in the veins. *P < .05 compared with control at the same time-point. Journal of Vascular Surgery 2010 52, 1310-1320DOI: (10.1016/j.jvs.2010.05.095) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 2 Transient receptor potential vanilloid channel (TRPV) expression in veins. A, Representative blots showing TRPV1 and TRPV4 protein levels in the vein (n = 2). The bar graphs show a densitometric quantitation of the TRPV to actin ratio. B, RT-PCR analysis of changes in TRPV1 and TRPV4 mRNA in control (C) and fistula veins at various times. C, TRPV1 (red) is present in endothelium of the control and 7- and 14-day fistula veins. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. D, Linear regression analysis of wall shear stress (WSS) and TRPV1 protein levels in veins. E and F, Vessel restriction by ligature reduces blood flow and WSS (E) as well as TRPV1 protein levels (F). N = 6 in all bar graphs. *P < .05 compared with control (at the same time-point in A). †P < .05 compared with the untreated fistula vein. Journal of Vascular Surgery 2010 52, 1310-1320DOI: (10.1016/j.jvs.2010.05.095) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 3 Changes in enzymes controlling nitric oxide (NO) production and metalloproteinases (MMP)2 activation in veins. A, Western blot shows the expression of phosphorylated and total CaMK II. The bar graph shows the ratio of the two proteins and represents CaMK II activity. B, Expression of eNOS and iNOS in veins. AU, Arbitrary units of band density. C, Upper panels show that eNOS (green) is present in the veins, and lower panels show the colocalization (yellow in merge picture) of transient receptor potential vanilloid channel 1 (TRPV1) (red) and eNOS (green) in endothelium of a 28-day fistula vein. Nuclei are stained with DAPI (blue). Scale bar: 100 μm. D, Expression of arginase I and II in veins. E, A specific blocker of CaMK II, KN-93, reverses eNOS and arginase I expression in the 28-day fistula vein. F, Content of two NO metabolites, nitrate and nitrite (NOx), in veins. G, Zymography reveals that both the proenzyme and active forms of MMP2 have increased activity in the fistula veins. N = 6 in all bar graphs. *P < .05 compared with the control. †P < .05 compared with the untreated fistula vein. Journal of Vascular Surgery 2010 52, 1310-1320DOI: (10.1016/j.jvs.2010.05.095) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 4 Capsazepine attenuates remodeling. A, Hemodynamic changes in veins. B, Representative images and quantitation of changes in the thickness of intima and media, and the ratio of intima to media (IM) in the venous wall. Nuclei are stained by hematoxylin (blue). Scale bar: 20 μm. N = 6 in all bar graphs. *P < .05 compared with the control veins (C). †P < .05 compared with the untreated fistula vein. Journal of Vascular Surgery 2010 52, 1310-1320DOI: (10.1016/j.jvs.2010.05.095) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 5 Effects of transient receptor potential vanilloid channel 1 (TRPV1) blockade. A through C, Western blots showing TRPV1 expression, CaMK II activity, or eNOS and arginase I expression in veins in the presence or absence of capsazepine treatment. D, Nitric oxide (NO) metabolites (NOx) in the veins. E, Zymography revealing changes in the activity of both the proenzyme and the active form of metalloproteinases (MMP)2 in the veins. N = 6 in all bar graphs. *P < .05 compared to the control veins (C). †P < .05 compared with the untreated fistula vein. Journal of Vascular Surgery 2010 52, 1310-1320DOI: (10.1016/j.jvs.2010.05.095) Copyright © 2010 Society for Vascular Surgery Terms and Conditions

Fig 6 Model showing how transient receptor potential vanilloid channel 1 (TRPV1) contributes to high blood flow-induced venous remodeling. Journal of Vascular Surgery 2010 52, 1310-1320DOI: (10.1016/j.jvs.2010.05.095) Copyright © 2010 Society for Vascular Surgery Terms and Conditions