Next-Generation Sequencing for Simultaneous Determination of Human Papillomavirus Load, Subtype, and Associated Genomic Copy Number Changes in Tumors  Caroline Conway, Rebecca Chalkley, Alec High, Kenneth Maclennan, Stefano Berri, Preetha Chengot, Melissa Alsop, Philip Egan, Joanne Morgan, Graham R. Taylor, John Chester, Mehmet Sen, Pamela Rabbitts, Henry M. Wood  The Journal of Molecular Diagnostics  Volume 14, Issue 2, Pages 104-111 (March 2012) DOI: 10.1016/j.jmoldx.2011.10.003 Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 The statistically detectable viral load for increasing numbers of reads. The lines indicate the minimum detectable numbers of viruses per cell for different read numbers for three different probabilities. The Journal of Molecular Diagnostics 2012 14, 104-111DOI: (10.1016/j.jmoldx.2011.10.003) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Frequency of genomic gain and loss for HPV-positive and HPV-negative tumor groups. For each position along the genome, the proportion of patient tumor samples with a copy number log2 ratio of ±0.25 is measured and displayed as frequency of gain (red) or loss (green). Here, patient tumor samples with HPV detected by sequencing (NGS HPV-positive, n = 10) are compared to tumors with no HPV sequence detected (NGS HPV-negative, n = 21). The Journal of Molecular Diagnostics 2012 14, 104-111DOI: (10.1016/j.jmoldx.2011.10.003) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions