Immunoadsorption of anti-Rpp25 antibodies.

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Immunoadsorption of anti-Rpp25 antibodies. Immunoadsorption of anti-Rpp25 antibodies. The first serum sample of the patient was incubated with paramagnetic beads coupled with recombinant Rpp25 antigen to immunoadsorb antibodies and then tested on QUANTA Flash CTD Screen Plus. As control, beads coupled with recombinant centromere protein B (CENP-B) were used. Inhibition was observed with Rpp25, but not with CENP-B, indicating that anti-Rpp25 antibodies are the only detectable antibody in the first serum sample of the patient. Antigens contained in the QUANTA Flash CTD Screen Plus: dsDNA, Sm/RNP, Ro52, Ro60, SS-B, Scl-70, CENP, Mi-2, Ku, Th/To, RNA Pol III, Pm/Scl, PCNA, Jo-1, and ribosomal-P protein. CTD: connective tissue disease; CU: chemiluminescence units. MARTIAL KOENIG et al. J Rheumatol 2017;44:1938-1939 ©2017 by The Journal of Rheumatology