Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia Kewal Asosingh, PhD, Jodi D.

Slides:

Advertisements

Similar presentations

Kyoung-Bok Min, MD, PhD, Jin-Young Min, PhD

Advertisements

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy Yin Yao, MD, Cai-Ling.

The effects of upper respiratory infection on T-cell proliferation and steroid sensitivity of asthmatics Elcio O. Vianna, MD, PhD, Jay Westcott, PhD,

IL-4 induces IL-13–independent allergic airway inflammation

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IL-25 and CD4+ TH2 cells enhance type 2 innate lymphoid cell–derived IL-13 production, which promotes IgE-mediated experimental food allergy Jee-Boong.

Ann-Marie M. Schoos, MD, PhD, Jacob D

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma Tobias Polte, PhD, Christian.

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma Frank Kirstein, PhD, William G.C.

Frank Kirstein, PhD, Natalie E

Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model Roman Karwot, PhD, Joachim H. Maxeiner,

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels Miguel L. Stein, MD, Joyce M. Villanueva,

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,

Group 2 innate lymphoid cells facilitate sensitization to local, but not systemic, TH2- inducing allergen exposures Matthew J. Gold, BSc, Frann Antignano,

Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.

Dorothy M. Ryan, MD, Stephen J. Fowler, MD, Robert M. Niven, MD

Is 9 more than 2 also in allergic airway inflammation?

Responsiveness to respiratory syncytial virus in neonates is mediated through thymic stromal lymphopoietin and OX40 ligand Junyan Han, PhD, Azzeddine.

CD4+ T cells migrate from airway to bone marrow after antigen inhalation in rats Susumu Isogai, MD, Shigeru Miyata, MD, Rame Taha, MD, Yasuyuki Yoshizawa,

Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Activin A and TGF-β promote TH9 cell–mediated pulmonary allergic pathology Carla P. Jones, PhD, Lisa G. Gregory, PhD, Benjamin Causton, BSc, Gaynor A.

Human mast cells arise from a common circulating progenitor

Prime role of IL-17A in neutrophilia and airway smooth muscle contraction in a house dust mite–induced allergic asthma model Julie Chesné, PhD, Faouzi.

CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling Jennifer Kearley, PhD, Douglas S. Robinson,

RhoA signaling through platelet P2Y1 receptor controls leukocyte recruitment in allergic mice Richard T. Amison, MSci, Stefania Momi, PhD, Abigail Morris,

Experimental gastrointestinal allergy enhances pulmonary responses to specific and unrelated allergens Eric B. Brandt, PhD, Troy A. Scribner, MD, Hiroko.

Frank Kirstein, PhD, Natalie E

Ton C. Doan, BA, Brian M. Jeong, MSc, Mackenzie E. Coden, BA, Lucas F

Thymic stromal lymphopoietin activation of basophils in patients with allergic asthma is IL-3 dependent Brittany M. Salter, BSc, John Paul Oliveria,

Targeting Fel d 1 to FcγRI induces a novel variation of the TH2 response in subjects with cat allergy Kathryn E. Hulse, BS, Amanda J. Reefer, MS, Victor.

Jethe O. F. Nunes, PhD, Juliana de Souza Apostolico, MSc, David A. G

Janus kinase 1/3 signaling pathways are key initiators of TH2 differentiation and lung allergic responses Shigeru Ashino, PhD, Katsuyuki Takeda, MD,

Β2 integrins rather than β1 integrins mediate Alternaria-induced group 2 innate lymphoid cell trafficking to the lung Maya R. Karta, PhD, Peter S. Rosenthal,

Floor Weerkamp, MSc, Edwin F. E. de Haas, BSc, Brigitta A. E

Brent E. Palmer, PhD, Douglas G. Mack, PhD, Allison K

Role of B cells in TH cell responses in a mouse model of asthma

Decreases in human dendritic cell–dependent TH2-like responses after acute in vivo IgE neutralization John T. Schroeder, PhD, Anja P. Bieneman, BS, Kristin.

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Marc C. Levesque, MD, PhD, E. William St. Clair, MD

Myeloid and plasmacytoid dendritic cells in induced sputum after allergen inhalation in subjects with asthma Benny Dua, MSc, Richard M. Watson, BSc,

Increased frequency of dual-positive TH2/TH17 cells in bronchoalveolar lavage fluid characterizes a population of patients with severe asthma Chaoyu.

Cysteinyl leukotrienes acting via granule membrane-expressed receptors elicit secretion from within cell-free human eosinophil granules Josiane S. Neves,

Staphylococcal enterotoxin A–activated regulatory T cells promote allergen-specific TH2 response to intratracheal allergen inoculation Wei-ping Zeng,

What is an “eosinophilic phenotype” of asthma?

Xin-Zi Tang, PhD, James B. Jung, BS, Christopher D.C. Allen, PhD

Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent Sharen Provoost, MSc, Tania.

Autophagy: Nobel Prize 2016 and allergy and asthma research

Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity Iris Bellinghausen, PhD, Sebastian.

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Effect of aging on sputum inflammation and asthma control

Rhinovirus infection interferes with induction of tolerance to aeroantigens through OX40 ligand, thymic stromal lymphopoietin, and IL-33 Amit K. Mehta,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Ozone activates pulmonary dendritic cells and promotes allergic sensitization through a Toll-like receptor 4–dependent mechanism John W. Hollingsworth,

Lung type 2 innate lymphoid cells express cysteinyl leukotriene receptor 1, which regulates TH2 cytokine production Taylor A. Doherty, MD, Naseem Khorram,

Blocking IL-25 prevents airway hyperresponsiveness in allergic asthma

David S. Southam, BSc, Natasha Widmer, Russ Ellis, BSc, Jeremy A

Clinical safety of Food Allergy Herbal Formula-2 (FAHF-2) and inhibitory effect on basophils from patients with food allergy: Extended phase I study

MicroRNA-155 is essential for TH2-mediated allergen-induced eosinophilic inflammation in the lung Carina Malmhäll, BSc, Sahar Alawieh, BSc, You Lu, PhD,

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

CD11b-mediated migratory property of peripheral blood B cells

Epicutaneous immunization with ovalbumin and CpG induces TH1/TH17 cytokines, which regulate IgE and IgG2a production Monika Majewska-Szczepanik, PhD,

IgG4 production is confined to human IL-10–producing regulatory B cells that suppress antigen-specific immune responses Willem van de Veen, MSc, Barbara.

Systemic responses after bronchial aspirin challenge in sensitive patients with asthma Joanna S. Makowska, MD, PhD, Janina Grzegorczyk, PhD, Barbara Bienkiewicz,

Local treatment with IL-12 is an effective inhibitor of airway hyperresponsiveness and lung eosinophilia after airway challenge in sensitized mice Jürgen.

Natural history of cow’s milk allergy

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Presentation transcript:

Allergen-induced, eotaxin-rich, proangiogenic bone marrow progenitors: A blood-borne cellular envoy for lung eosinophilia Kewal Asosingh, PhD, Jodi D. Hanson, MS, Georgiana Cheng, MD, Mark A. Aronica, MD, Serpil C. Erzurum, MD Journal of Allergy and Clinical Immunology Volume 125, Issue 4, Pages 918-925 (April 2010) DOI: 10.1016/j.jaci.2010.01.017 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Expression of eotaxin by bone marrow–derived EPCs. A, EPCs were isolated from the bone marrow and characterized for the expression of endothelial, progenitor cell, and myeloid markers. Dotted lines indicate autofluorescence and background staining. B, Eotaxin-1 expression by EPCs in different experimental conditions. Bar = 200 μm. Journal of Allergy and Clinical Immunology 2010 125, 918-925DOI: (10.1016/j.jaci.2010.01.017) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Secretion of eotaxin-1 in cocultures of EPCs and lung vascular cells. A, Isolation of ECs and SMCs from lungs. Dot plots of CD31+ cells before and after purification are shown. Histograms for α-SMC actin (solid line) and calponin (solid line) expression in SMCs are shown. Dashed lines indicate staining with control antibodies. Phase-contrast images of the ECs and SMCs are also shown. Eotaxin-1 secretion by EPCs (B), lung ECs (C), and lung SMCs (D) and cocultures of EPCs with lung ECs (E), EPCs with lung SMCs (F), and EPCs with heart and liver vascular cells (G) are shown. Data represent mean ± SE values of 6 mice in each group. IP, Intraperitoneal. Journal of Allergy and Clinical Immunology 2010 125, 918-925DOI: (10.1016/j.jaci.2010.01.017) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Recruitment of eosinophils into the lungs by EPC-conditioned medium. EPC-conditioned supernatants (eotaxin-1 concentration of approximately 400 pg/mL) were intranasally administered into mice that had undergone sham (−) or OVA intraperitoneal (IP) sensitization. Eosinophils in the peripheral blood circulation and recruitment into lungs were analyzed by means of flow cytometry. Preincubation of the conditioned medium with neutralizing eotaxin-1 antibody was performed to block eotaxin-1. Isotype-matched control antibodies had no significant effects (data not shown). Data represent mean ± SE values from 3 mice in each group. Journal of Allergy and Clinical Immunology 2010 125, 918-925DOI: (10.1016/j.jaci.2010.01.017) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Eotaxin-1–positive EPCs in allergen-exposed lungs. A, Mononuclear cells were isolated from lungs and evaluated for eotaxin-1 expression. Representative values for 3 mice in each group are shown. B, Eotaxin-1 content per positive cells quantified with Image Pro software. IP, Intraperitoneal. Journal of Allergy and Clinical Immunology 2010 125, 918-925DOI: (10.1016/j.jaci.2010.01.017) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Expression of eotaxin-1 by circulating human EPCs. A, Eotaxin-1 expression analyzed by means of immunofluorescence staining. Bar = 200 μm. B, Percentage of eotaxin-1–positive EPCs. C, Eotaxin-1 content per positive cell was quantified with Image Pro software. Median values and upper and lower quartiles are shown. Journal of Allergy and Clinical Immunology 2010 125, 918-925DOI: (10.1016/j.jaci.2010.01.017) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions