CD203c expression on human basophils is associated with asthma exacerbation Emiko Ono, MD, PhD, Masami Taniguchi, MD, PhD, Noritaka Higashi, MD, PhD,

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Presentation transcript:

CD203c expression on human basophils is associated with asthma exacerbation Emiko Ono, MD, PhD, Masami Taniguchi, MD, PhD, Noritaka Higashi, MD, PhD, Haruhisa Mita, PhD, Keiichi Kajiwara, Hiromichi Yamaguchi, MD, Sayaka Tatsuno, MD, Yuma Fukutomi, MD, Hidenori Tanimoto, MD, Kiyoshi Sekiya, MD, Chiyako Oshikata, MD, Takahiro Tsuburai, MD, PhD, Naomi Tsurikisawa, MD, PhD, Mamoru Otomo, MD, PhD, Yuji Maeda, MD, Maki Hasegawa, MD, PhD, Eishi Miyazaki, MD, PhD, Toshihide Kumamoto, MD, PhD, Kazuo Akiyama, MD Journal of Allergy and Clinical Immunology Volume 125, Issue 2, Pages 483-489.e3 (February 2010) DOI: 10.1016/j.jaci.2009.10.074 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Relationship between asthma control and expression levels of CD63, CD69, and CD203c on basophils. Comparison of the expression levels of CD63, CD69, and CD203c on basophils from patients with asthma exacerbation (BA-exacerbation; n = 24), patients with stable asthma (BA-stable; n = 16), and healthy controls (HC; n = 11). The spontaneous and anti-IgE–induced expression levels are shown in 1 and 2, respectively. Data are expressed as net MFI, and error bars indicate SEMs. Journal of Allergy and Clinical Immunology 2010 125, 483-489.e3DOI: (10.1016/j.jaci.2009.10.074) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Changes in CD203c expression levels in on basophils from patients with asthma exacerbation after therapy. Changes in spontaneous (A) and anti-IgE–induced (B) expression levels of CD203c in the patients with asthma exacerbation, before and after therapy. NS, Not significant. Journal of Allergy and Clinical Immunology 2010 125, 483-489.e3DOI: (10.1016/j.jaci.2009.10.074) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 CD203c expression after in vitro stimulation of basophils with Der p 1 or IL-3. CD203c expression levels induced by in vitro stimulation of basophils (from patients with asthma exacerbation or clinical improvement) with various concentrations of Der p 1 (1, n = 14) or IL-3 (2, n = 24). ∗P <.05, ∗∗P <.001 compared with the CD203c expression levels for the same concentrations in the patients with asthma exacerbation. Journal of Allergy and Clinical Immunology 2010 125, 483-489.e3DOI: (10.1016/j.jaci.2009.10.074) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Relationship between expression levels of CD203c on basophils and FEV1 in pulmonary function tests. In patients with clinical improvement, spontaneous CD203c expression levels correlated with the percent predicted values for FEV1. Journal of Allergy and Clinical Immunology 2010 125, 483-489.e3DOI: (10.1016/j.jaci.2009.10.074) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Example of optimal basophil gating in the CD203c protocol (A) and induced CD203c expression by stimulation with anti-IgE, Der p 1, IL-3, or 15R-MePGD2 (B). Basophils were detected on the basis of forward side scatter characteristics and negative expression of CD3 and positive expression of CRTH2. In each assay, isotype control was used for negative staining. Data were expressed as net MFI (actual MFI – MFI of isotype IgG control). Journal of Allergy and Clinical Immunology 2010 125, 483-489.e3DOI: (10.1016/j.jaci.2009.10.074) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Induced CD203c expression by in vitro stimulation with 15R-MePGD2 Induced CD203c expression by in vitro stimulation with 15R-MePGD2. No significant difference was found between the patients with asthma exacerbation and those with clinical improvement in the extent of CD203c expression induced by 15R-MePGD2. We examined the effect of 15R-MePGD2 on CD203c expression in both the atopic (n = 14) and nonatopic (n = 10) patients with asthma. Journal of Allergy and Clinical Immunology 2010 125, 483-489.e3DOI: (10.1016/j.jaci.2009.10.074) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions