Baby bottle steam sterilizers disinfect home nebulizers inoculated with bacterial respiratory pathogens  Dana Towle, Deborah A. Callan, Patricia A. Farrel,

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Baby bottle steam sterilizers disinfect home nebulizers inoculated with bacterial respiratory pathogens  Dana Towle, Deborah A. Callan, Patricia A. Farrel, Marie E. Egan, Thomas S. Murray  Journal of Cystic Fibrosis  Volume 12, Issue 5, Pages 512-516 (September 2013) DOI: 10.1016/j.jcf.2012.11.013 Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

Fig. 1 Bacterial inoculation sites. The nebulizers Pari LC Plus® (A) and eFlow®rapid (B) were disassembled for inoculation and then reassembled prior to steam treatment for experiments after the initial comparison of assembled and disassembled steam treatments. The Altera® was inoculated as shown for the eFlow® rapid. Journal of Cystic Fibrosis 2013 12, 512-516DOI: (10.1016/j.jcf.2012.11.013) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions

Fig. 2 Pretreatment growth of bacteria from de-identified pooled CF sputum inoculated with P. aeruginosa. The chocolate agar (A) and blood agar plates (C) show both the laboratory inoculated P. aeruginosa and the mixed normal flora. The colistin nalidixic acid agar plate (B) that selects for gram positive organisms shows only mixed normal flora (e.g. white colonies) and no P. aeruginosa while the MacConkey agar plate (D) that is selective for gram negative bacterium shows only P. aeruginosa. No bacteria were recovered after steam treatment from any plates. Journal of Cystic Fibrosis 2013 12, 512-516DOI: (10.1016/j.jcf.2012.11.013) Copyright © 2012 European Cystic Fibrosis Society. Terms and Conditions