Overexpression of Transcription Factor Ovol2 in Epidermal Progenitor Cells Results in Skin Blistering Briana Lee, Kazuhide Watanabe, Daniel Haensel,

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Overexpression of Transcription Factor Ovol2 in Epidermal Progenitor Cells Results in Skin Blistering Briana Lee, Kazuhide Watanabe, Daniel Haensel, Jennifer Y. Sui, Xing Dai Journal of Investigative Dermatology Volume 137, Issue 8, Pages 1805-1808 (August 2017) DOI: 10.1016/j.jid.2017.02.985 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Skin blistering and basal cytolysis in Ovol2 BT mice. (a) Ovol2 BT newborns display shiny skin with blisters. (b) Histologic analysis of newborn skin. (c) Barrier assay of newborns. Arrow indicates dye uptake at a blistered site. (d) Cytolysis of the basal cells. (e) TUNEL-positive cells are present in newborn BT epidermis where blistering occurs. EM images of (f, i) WT and (g, j) BT epidermal cells. White arrow indicates the keratin ring. (h) Diagram of normal hemidesmosomal structure. Scale bar = 50 μm. B, basal cell; BT, bitransgenic; EM, electron microscopy; H, hemidesmosome; K, keratin filaments; LD, lamina densa; Pq, plaque; Pt, inner plate; S, spinous cell; TG, single transgenic control; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; WT, wild-type. Journal of Investigative Dermatology 2017 137, 1805-1808DOI: (10.1016/j.jid.2017.02.985) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Ovol2 overexpression causes reduced basal keratin expression. (a) Microarray and (b) RT-PCR analysis revealing altered keratin mRNA levels in embryonic BT skin. P < 0.002 in (a). (c–f) Protein expression analysis of E18.5 skin using the indicated antibodies. DAPI stains the nuclei. (g) Keratin gene expression in MCF10A cells. (h) Semiquantitative RT-PCR revealing the decreased AP-2γ mRNA level in BT skin. (i) Ovol2 binds to the AP-2γ promoter in mouse skin. Scale bar = 50 μm. BT, bitransgenic; Neg, negative control; RT-PCR, reverse transcriptase-PCR; TG, single transgenic control; WT, wild-type. Journal of Investigative Dermatology 2017 137, 1805-1808DOI: (10.1016/j.jid.2017.02.985) Copyright © 2017 The Authors Terms and Conditions