A 39-bp Deletion Polymorphism in PTEN in African American Individuals

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Presentation transcript:

A 39-bp Deletion Polymorphism in PTEN in African American Individuals Xiao-Ping Zhou, Heather Hampel, Jennifer Roggenbuck, Nabil Saba, Thomas W. Prior, Charis Eng The Journal of Molecular Diagnostics Volume 4, Issue 2, Pages 114-117 (May 2002) DOI: 10.1016/S1525-1578(10)60690-3 Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Characterization of an intronic 39-bp deletion polymorphism in PTEN. a: PCR products were electrophoresed on 2% agarose gel with 100-bp DNA ladder. Arrows denote samples which are heterozygous for the 39-bp deletion. b:Fluorescent sizing of the 39-bp deletion. The upper panelshows a wild-type homozygote for the full-length 322-bp fluorescence-labeled PCR product. The lower panel shows a heterozygote with one allele containing the 39-bp deletion, which resulted in a short product of 284 bp, and the wild-type allele resulting in a full-length 322-bp product. c: Sequencing of the variant (upper panel) and the full-length (lower panel) PCR products revealed the 39-bp intronic deletion (IVS7–15→53). The deleted nucleotides are underlined in the lower panel. The Journal of Molecular Diagnostics 2002 4, 114-117DOI: (10.1016/S1525-1578(10)60690-3) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions