Pregnancy and live birth following the transfer of vitrified–warmed blastocysts derived from zona- and corona-cell-free oocytes  Yimin Shu, Wenlin Peng,

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Pregnancy and live birth following the transfer of vitrified–warmed blastocysts derived from zona- and corona-cell-free oocytes  Yimin Shu, Wenlin Peng, John Zhang  Reproductive BioMedicine Online  Volume 21, Issue 4, Pages 527-532 (October 2010) DOI: 10.1016/j.rbmo.2010.04.033 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Embryonic development after zona pellucida reconstruction in case 1. (A) Normally fertilized zona-intact and zona-free oocytes. (B) A 4-cell-stage zona-intact embryo and a zona-free embryo with loosely associated blastomeres on day 2. (C) Reconstructed embryo after insertion of blastomeres into a ruptured zona pellucida. (D) Hatching blastocyst on day 6. Bars=50μm. Reproductive BioMedicine Online 2010 21, 527-532DOI: (10.1016/j.rbmo.2010.04.033) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Embryonic development of a zona-free oocyte (case 2). (A) Fertilized zona-free oocyte. (B) A 2-cell-stage zona-free embryo with tight contact between blastomeres on day 2. (C) Compacted zona-free embryo on day 3. (D) Zona-free blastocyst on day 5. Bars=50μm. Reproductive BioMedicine Online 2010 21, 527-532DOI: (10.1016/j.rbmo.2010.04.033) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

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