Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. Virus-specific serum antibody response of BALB/c mice to DNA.

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Presentation transcript:

Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. IgG1 and IgG2a antibody isotype titers against HK/Syd (1 μg HA ml−1) were measured by ELISA and are shown after primary (day 14), secondary (day 14), tertiary (day 21), and quaternary (day 21) responses to the vaccine. For all days and isotypes measured, the following numbers of mice were included in the various groups: for vector DNA plus PBS, n = 8; for vector DNA plus GFP-VRP, n = 15; for vector DNA plus HA-VRP, n = 15; for HA-DNA plus PBS, n = 10; for HA-DNA plus GFP-VRP, n = 17; and for HA-DNA plus HA-VRP, n = 18. An asterisk indicates a significant difference in titer for HA-DNA-vaccinated mice compared to that for vector DNA-vaccinated mice (P < 0.01 by ANOVA). A double asterisk indicates a significant difference in titer for mice vaccinated with HA-DNA plus HA-VRP compared to those for all other groups (P < 0.01 by ANOVA). Victor C. Huber et al. Clin. Vaccine Immunol. 2006; doi:10.1128/CVI.00156-06