Heterogeneous Staining for Mismatch Repair Proteins during Population-Based Prescreening for Hereditary Nonpolyposis Colorectal Cancer Natasha Watson,

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Heterogeneous Staining for Mismatch Repair Proteins during Population-Based Prescreening for Hereditary Nonpolyposis Colorectal Cancer Natasha Watson, Fabienne Grieu, Melinda Morris, Jennet Harvey, Colin Stewart, Lyn Schofield, Jack Goldblatt, Barry Iacopetta The Journal of Molecular Diagnostics Volume 9, Issue 4, Pages 472-478 (September 2007) DOI: 10.2353/jmoldx.2007.060162 Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Representative F-SSCP gel showing deletions in the BAT-26 mononucleotide repeat indicative of the MSI+ phenotype. Samples on the left are cases in which earlier detection of MSI+ status was independently confirmed by separate PCR and SSCP gel run. The Journal of Molecular Diagnostics 2007 9, 472-478DOI: (10.2353/jmoldx.2007.060162) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Heterogeneous IHC staining patterns for MSI+ CRC. A: Zonal loss for PMS2. B: Intraglandular heterogeneity for MSH6. The Journal of Molecular Diagnostics 2007 9, 472-478DOI: (10.2353/jmoldx.2007.060162) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Proposed flowchart for the population-based detection of HNPCC based on routine screening for MSI as the first test. The Journal of Molecular Diagnostics 2007 9, 472-478DOI: (10.2353/jmoldx.2007.060162) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions