A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR 

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A comparison of three real-time PCR assays for the confirmation of Neisseria gonorrhoeae following detection of N. gonorrhoeae using Roche COBAS AMPLICOR  L. Chui, T. Chiu, J. Kakulphimp, G.J. Tyrrell  Clinical Microbiology and Infection  Volume 14, Issue 5, Pages 473-479 (May 2008) DOI: 10.1111/j.1469-0691.2008.01950.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 (a) Amplified products (260 bp) generated by PCR using the orf1 gene as target. (b) HindIII fragments (107 bp and 153 bp) generated from the PCR products shown in (a). Lanes: 1, 8 and 15, 100-bp marker; 2–7 and 9–12, clinical isolates; 13, Neisseria gonorrhoeae positive control strain ATCC 49226; 14, negative control (water). Clinical Microbiology and Infection 2008 14, 473-479DOI: (10.1111/j.1469-0691.2008.01950.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

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