A Conserved Sequence Motif in Polycomb-Response Elements

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A Conserved Sequence Motif in Polycomb-Response Elements Jozsef Mihaly, Rakesh K. Mishra, François Karch Molecular Cell Volume 1, Issue 7, Pages 1065-1066 (June 1998) DOI: 10.1016/S1097-2765(00)80107-0

Figure 1 A Conserved Sequence Motif in Polycomb-Response Elements Position 1 refers to the first nucleotide in the YY1 consensus shown at the bottom of the figure. All of the PREs listed fulfill at least 1 of 3 criteria used to define PREs. In the first criterion, a PRE is identified by its ability to maintain a pattern of expression of a lacZ reporter gene throughout development (maintenance assay). The second assay (pairing-sensitive assay) is based on the property of a PRE to repress expression of a mini-white reporter gene. Finally, in the third assay (salivary gland assay), a PRE is identified by the creation of an additional chromosomal binding site of the PcG-repressing complex. The first 8 rows show the conserved motif found in 6 PREs from the BX-C. The sequences have been extracted from the sequence of the whole BX-C (Martin et al. 1995). The bxd PRE harbors all three characteristics (maintenance, pairing-sensitive, and salivary gland assays; Muller and Bienz 1991; Simon et al. 1993; Chan et al. 1994; Chiang et al. 1995). The iab-2 PRE contains two homology boxes (a and b) and has been identified in the maintenance and pairing-sensitive assays (J. Burr, M. J. O'Connor, and M. O'Connor, personal communication). The Mcp PRE has been extensively characterized in the pairing-sensitive assay (M. Müller and P. Schedl, personal communication) and in the salivary gland assay (Chiang et al. 1995). The iab-6 PRE has been identified in the pairing-sensitive assay (K. Hagstrom and P. Schedl, personal communication). The iab-7 PRE contains two homology motifs, a and b. This PRE has been characterized in all three assays (Zink and Paro 1995; Hagstrom et al. 1997; Mihaly et al. 1997). The iab-8 PRE has been identified in the maintenance assay (Barges and F. K., unpublished data) and in the pairing-sensitive assay (M. Müller and P. Schedl, personal communication). The conserved sequence motif found in three PREs from the Scr regulatory regions is also shown. These PREs have been identified in the pairing-sensitive assay (Gindhart and Kaufman 1995), and their sequences have been extracted after assembling three sequence contigs deposited in the GenBank database by the Berkeley Drosophila Genome Project (AC002512, AC001654, and AC001653). The sequence motif found in two PREs from the engrailed regulatory region is also shown. These PREs have been characterized in the pairing-sensitive assay (Kassis 1994). The en PHO/YY1 sequence is the element described by Brown et al. 1998 in this issue of Molecular Cell. Finally, the sequence motif found in the PRE from the regulatory region of polyhomeotic is also shown. This PRE has been identified in the pairing-sensitive and salivary gland assays (Fauvarque and Dura 1993). Molecular Cell 1998 1, 1065-1066DOI: (10.1016/S1097-2765(00)80107-0)