Detection of chromosomal blaCTX-M-2 in diverse Escherichia coli isolates from healthy broiler chickens J.C. Ferreira, R.A.C. Penha Filho, L.N. Andrade,

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Detection of chromosomal blaCTX-M-2 in diverse Escherichia coli isolates from healthy broiler chickens J.C. Ferreira, R.A.C. Penha Filho, L.N. Andrade, A. Berchieri, A.L.C. Darini Clinical Microbiology and Infection Volume 20, Issue 10, Pages O623-O626 (October 2014) DOI: 10.1111/1469-0691.12531 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Dendrogram of genomic similarity obtained by XbaI-PFGE of 19 ESBL-producing Escherichia coli isolates from broiler chickens. NAL, nalidixic acid; CIP, ciprofloxacin; LVX, levofloxacin; TET, tetracycline; GEN, gentamicin; SXT, trimethoprim/sulphamethoxazole; CHL, chloramphenicol. Clinical Microbiology and Infection 2014 20, O623-O626DOI: (10.1111/1469-0691.12531) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Digested fragments of the genomic DNA of CTX-M-2-producing Escherichia coli isolates representing the diverse PFGE-types detected. (a) PFGE profiles using the I-Ceu-I enzyme. (b) Southern blot and hybridization with blaCTX-M-2 probe after I-Ceu-I-PFGE. (c) Southern blot and hybridization with probe for E. coli 16S rRNA gene after I-Ceu-I-PFGE. Clinical Microbiology and Infection 2014 20, O623-O626DOI: (10.1111/1469-0691.12531) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions