Robustness of the cellular system in sake yeast strains.

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Robustness of the cellular system in sake yeast strains. Robustness of the cellular system in sake yeast strains. (A) Distribution of phenotypic noise among the sake strains. Yellow boxes indicate the sake yeasts in which phenotypic noise was significantly higher than that of the K7 strain (P < 0.05, Dunnett’s test). The bottom and top of the box indicate the first and third quantiles, respectively. The band inside the box indicates the median. The bottom and top of the whiskers indicate the minimum and the maximum, respectively. (B) Sensitivity of the sake yeast strains to various robustness-disrupting stressors. Yeast strains were cultured in liquid YPD medium, and diluted serially to 1 × 107, 1 × 106, 1 × 105, 1 × 104, and 1 × 103 cells/ml. 5-μl drops were spotted on plates containing benomyl (10 μg/ml), hydroxyurea (HU) (100 mM), calcofluor white (CW) (50 μg/ml), or DMSO (0.2%) as a control. Consequently, 5 × 104, 5 × 103, 5 × 102, 5 × 101, and 5 cells were plated in each spot. The plates were incubated at 25° for 5 d. Shinsuke Ohnuki et al. G3 2017;7:2807-2820 ©2017 by Genetics Society of America