Action of mifepristone on the expression of insulin-like growth factor binding protein-1 mRNA and protein during the early luteal phase in the human oviduct

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Presentation transcript:

Action of mifepristone on the expression of insulin-like growth factor binding protein-1 mRNA and protein during the early luteal phase in the human oviduct Xiaoyan Qiu, M.D., Ph.D., Xiaoxi Sun, M.D., Alexander Christow, M.D., Berit Ståbi, B.M.A., Kristina Gemzell-Danielsson, M.D., Ph.D. Fertility and Sterility Volume 80, Pages 776-782 (September 2003) DOI: 10.1016/S0015-0282(03)00781-7

FIGURE 1 Immunohistochemical localization of IGFBP-1 in the human fallopian tube and endometrium. (A) Immunoreative IGFBP-1 was localized primarily to the tubal epithelial cells. (B) Immunostaining for IGFBP-1 was detected in the subapical region of the epithelial cells. (C) Increased intensity of IGFBP-1 staining in the cytoplasm of all epithelial cells after treatment with mifepristone. (D) Note the staining for IGFBP-1 was apparently diffuse in the epithelial cells. (E) Moderate staining was observed in the endometrial gland. (F) Endometrial glandular cells sometimes also showed granular immunostaining. Bars = 100 μm. Qiu. IGFBP-1 in the human oviduct. Fertil Steril 2003. Fertility and Sterility 2003 80, 776-782DOI: (10.1016/S0015-0282(03)00781-7)

FIGURE 2 RT-PCR for IGFBP-1 (131 bp) and 28S (552 bp), using total RNA extracted from the fallopian tube samples. All samples were run at the same time. Lanes 1–4 represent controls and lanes 5–8 mifepristone treatment (ampullar [A] and isthmic [I]). Lane M is a 100-bp ladder; lane 9 is a negative control from which cDNA was omitted, and lane 10 is a positive control from endometrium. Qiu. IGFBP-1 in the human oviduct. Fertil Steril 2003. Fertility and Sterility 2003 80, 776-782DOI: (10.1016/S0015-0282(03)00781-7)

FIGURE 3 The expression of IGFBP-1 mRNA in the ampullar (A) and isthmic (I) part of human fallopian tubes treated with or without mifepristone on day LH+2. This increase was significant in both the ampullar and isthmic regions. Each column represents the mean of the intensity ratio. Bars show SD. *P<.05 compared with control group. OD = optical density. Qiu. IGFBP-1 in the human oviduct. Fertil Steril 2003. Fertility and Sterility 2003 80, 776-782DOI: (10.1016/S0015-0282(03)00781-7)