Volume 11, Issue 4, Pages 631-634 (April 2018) Highly Efficient A·T to G·C Base Editing by Cas9n-Guided tRNA Adenosine Deaminase in Rice  Fang Yan, Yongjie Kuang, Bin Ren, Jingwen Wang, Dawei Zhang, Honghui Lin, Bing Yang, Xueping Zhou, Huanbin Zhou  Molecular Plant  Volume 11, Issue 4, Pages 631-634 (April 2018) DOI: 10.1016/j.molp.2018.02.008 Copyright © 2018 The Authors Terms and Conditions

Figure 1 The rBE14 System Converts A to G Efficiently in the Rice Genome. (A) Constructs of the fluorescence-tracking rBE systems used to induce nucleotide changes in transgenic rice lines. (B) The target site of OsMPK6 gene in rice. (C) Representative Sanger sequencing chromatogram of the rBE14-edited OsMPK6 allele with the target A changed in T0 transgenic rice callus line #21. (D) The architecture of rice adenine base editors and its editing efficiencies at the OsMPK6 locus in rice. (E) The target site of OsSERK2 gene in rice. (F) Representative Sanger sequencing chromatogram of the rBE14-edited OsSERK2 allele with the target A changed in T0 transgenic rice callus line #9. (G) The target site of OsWRKY45 gene in rice. (H) Sequencing results of the rBE14-induced OsWRKY45 mutations in T0 transgenic rice callus lines. (I) Summary of nucleotide changes in the editing window of the endogenous OsWRKY45 gene in T0 transgenic rice callus lines. (J) Summary of base editing efficiencies of rBE14 with different DNA contexts at five genomic sites. (K) Visualizing GFP in transgenic rice callus under a handheld UV lamp and confocal microscope. For (B), (E), (G), (H), and (I), The PAM sequence, the candidate bases in the putative editing window, the detected nucleotide changes/the corresponding amino acids and the sgRNA sequence are highlighted in green, red, blue and bold, respectively. For (C) and (F), the nucleotide changes are underlined in the sequencing chromatograms. Molecular Plant 2018 11, 631-634DOI: (10.1016/j.molp.2018.02.008) Copyright © 2018 The Authors Terms and Conditions