Vincent L. Rowe, MD, Scott L. Stevens, MD, FACS, Tonya T

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Presentation transcript:

Vascular smooth muscle cell apoptosis in aneurysmal, occlusive, and normal human aortas Vincent L. Rowe, MD, Scott L. Stevens, MD, FACS, Tonya T. Reddick, MS, Michael B. Freeman, MD, FACS, Robert Donnell, DVM, PhD, ACVP, Roger C. Carroll, PhD, Mitchell H. Goldman, MD, FACS Journal of Vascular Surgery Volume 31, Issue 3, Pages 567-576 (March 2000) DOI: 10.1067/mva.2000.102847 Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 α1-Actin antibody staining. A , Normal aorta (NL ). Note the increased staining within the medial layer and the uniformity of the VSMC; the luminal surface is towards upper right hand corner. B , Occlusive aorta. Note the similar staining distribution; the luminal surface is towards the right. C , AAA. Note the disrupted architecture within the medial layer and the inflammatory cell and red blood cell infiltrate within the lower left corner ; the luminal surface is towards the upper right. (Original magnification of all tissue, ×100). AOD , Aortic occlusive disease. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 VSMC apoptosis staining in normal aorta. Cross-section of normal aortic tissue (original magnification, ×60). The arrow shows typical apoptotic VSMC within medial layer. L , Luminal surface. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 VSMC apoptosis staining in occlusive aorta. Cross-section of occlusive aortic tissue (original magnification, ×100). The arrow shows typical apoptotic VSMC within medial layer. Note the cholesterol plaque deposits on the lining of the endothelial layer. L , Luminal surface. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 VSMC apoptosis staining in aneurysmal aorta. Cross-section of aneurysmal aortic tissue (original magnification, ×100). The arrow denotes typical apoptotic VSMC within the medial layer. Nonlinear distribution of medial VSMC is apparent. L , Luminal surface. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 High-power VSMC apoptosis staining. Cross-section of medial layer AAA tissue sample (original magnification, ×400). The arrowhead is directed at positive nuclear staining for DNA end-labeling in apoptotic cells. Note the similar positive staining cells towards the periphery. The arrow points to the nonapoptotic cell. Note the lack of a dark brown–staining nucleus. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Colocalization of DNA end-labeling and α1-actin antibody. Cross-section of medial layer AAA tissue sample (original magnification, ×400). The hollow arrowhead shows a typical apoptotic nucleus with dark-brown staining. The solid arrowhead designates perinuclear pink staining for VSMC. The arrow shows a cell with positive staining with α1-actin antibody, but negative apoptosis staining. Note the disorganized array of VSMC orientation. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 7 PI assay. Cross-section of medial layer normal aorta tissue sample under rhodamine fluorescence (original magnification, ×400). The arrow shows apoptotic VSMC with fragmented, condensed nuclei and bright orange staining. Journal of Vascular Surgery 2000 31, 567-576DOI: (10.1067/mva.2000.102847) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions