575. High Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells Jared Carlson-Stevermer, Madelyn Goedland, Benjamin Steyer, Arezoo Movaghar,

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575. High Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells Jared Carlson-Stevermer, Madelyn Goedland, Benjamin Steyer, Arezoo Movaghar, Meng Lou, Lucille Kohlenberg, Ryan Prestil, Krishanu Saha Molecular Therapy Volume 24, Pages S229-S230 (May 2016) DOI: 10.1016/S1525-0016(16)33383-4 Copyright © 2016 The American Society of Gene & Cell Therapy Terms and Conditions

Figure ArrayEdit provides a new window into the process of gene editing human cells. Overview of ArrayEdit assembly and key components. Top: Schematic of one-pot PCR and T7 transcription. Bottom: Surface modification to the bottom of multiwell plates generates cell-adhesive µFeatures on a glass bottom. Each µFeature can be tracked over time via high-content imaging and stitched together to form a time-lapse visualization of edited cell phenotypes. Molecular Therapy 2016 24, S229-S230DOI: (10.1016/S1525-0016(16)33383-4) Copyright © 2016 The American Society of Gene & Cell Therapy Terms and Conditions