C. Candrian, S. Miot, F. Wolf, E. Bonacina, S. Dickinson, D. Wirz, M

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Presentation transcript:

Are ankle chondrocytes from damaged fragments a suitable cell source for cartilage repair? C. Candrian, S. Miot, F. Wolf, E. Bonacina, S. Dickinson, D. Wirz, M. Jakob, V. Valderrabano, A. Barbero, I. Martin Osteoarthritis and Cartilage Volume 18, Issue 8, Pages 1067-1076 (August 2010) DOI: 10.1016/j.joca.2010.04.010 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Histological appearance of detached fragments of OCLs of ankle joints (DACF) and NAC. Safranin-O (A, B), Von Kossa/Safranin-O staining (B), type II collagen and type I collagen immunohistochemical staining (A) of DACF harvested from two different, representative donors: donor 3 (I) and donor 5 (II) (see Table I for the donor information) and of NAC harvested from a cadaveric joint of a young individual (male, 32 years) (III). Bar=100μm. Arrows indicate cartilage/bone interface, asterisks show cell clusters, h=hyaline or hyaline-like cartilage, f=fibrocartilage, c=calcified cartilage. Osteoarthritis and Cartilage 2010 18, 1067-1076DOI: (10.1016/j.joca.2010.04.010) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Growth and differentiation stage of chondrocytes isolated from DACF and NAC. (A) Representative phase-contrast pictures of expanded DACF-chondrocytes (I) and NAC-chondrocytes (II) (see Table I for the donor information). Bar=100μm. (B) Proliferation rate of DACF- and NAC-chondrocytes. Values are the mean of measurements obtained from six different DACF or 10 different NAC; error bars represent the uncertainty with a 95%CI. (C) Real-time reverse transcriptase-polymerase chain reaction analysis of the expression of mRNA for type I (CI) and type II (CII) collagens (left y-axis labeling) or the CII/CI ratio (right y-axis labeling). Values are the mean of measurements obtained from four different DACF or four different NAC; error bars represent the uncertainty with a 95%CI. Osteoarthritis and Cartilage 2010 18, 1067-1076DOI: (10.1016/j.joca.2010.04.010) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Differentiation capacity of chondrocytes isolated from DACF and NAC in pellets. (A) Safranin-O stainings of representative pellets generated by DACF-chondrocytes harvested from two different donors: donor 3 (I), donor 5 (II) (see Table I for the donor information) and NAC-chondrocytes harvested from a cadaveric joint of a young individual (male, 32 years) (III) Bar=100μm. (B) Sulfate GAG content normalized to the amount of DNA in pellets. Values are the mean of triplicate pellets; error bars describe observed variations. The plotted lines indicate mean values measured in pellets generated by cells from NAC (upper line, 10 donors) or DACF (bottom line, six donors). (C) Type II collagen immunohistochemical stainings of representative pellets generated by DACF-chondrocytes harvested from two different donors: donor 2 (I), donor 3 (II) and NAC-chondrocytes harvested from a cadaveric joint of a young individual (male, 32 years) (III). Osteoarthritis and Cartilage 2010 18, 1067-1076DOI: (10.1016/j.joca.2010.04.010) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Differentiation capacity of chondrocytes isolated from DACF and NAC in HYAFF-11® scaffold. Safranin-O stainings (A) and type II collagen immunohistochemical stainings (B) of representative constructs generated by DACF-chondrocytes harvested from two different donors [donor 3 (I), donor 5 (II) (see Table I for the donor information)] and NAC-chondrocytes harvested from a cadaveric joint of a young individual (male, 32 years) (III) cultured in CHM for 28 days. Bar=100μm. h=hyaline-like cartilage, f=fibrocartilage (C) Amounts of sulfate GAG, type I collagen and type II collagen accumulated in constructs generated by DACF-chondrocytes (white bars, six donors) and NAC-chondrocytes (gray bars, 10 donors) expressed as a percentage of tissue dry weight; error bars represent the uncertainty with a 95%CI. (D) Equilibrium modulus and dynamic pulsatile modulus of constructs generated by DACF-chondrocytes (white bars, six donors) and NAC-chondrocytes (gray bars, 10 donors); error bars represent the uncertainty with a 95%CI. Osteoarthritis and Cartilage 2010 18, 1067-1076DOI: (10.1016/j.joca.2010.04.010) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions