The WD domain of ATG16L1 is not required for canonical autophagy

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The WD domain of ATG16L1 is not required for canonical autophagy The WD domain of ATG16L1 is not required for canonical autophagy ADiagram of full‐length (FL) 229–242 deletion (ΔFBD) and 1–336 (ΔWD) ATG16L1 constructs used in this study.B‐DWestern blot analysis of ATG16L1 in (B) HCT116 ATG16L1−/−, (C) MEF Atg16L1−/− and (D) MCF10A ATG16L1−/− cells stably re‐expressing ATG16L1 constructs. Arrows indicate specific ATG16L1 band.EWestern blotting for LC3 in complemented HCT116 cells ± PP242 (1 μM, 1 h).FQuantification of fold differences of LC3‐II/LC3‐I ratios over controls from (E).GConfocal images of GFP‐LC3 in complemented MEF cells ± starvation (1 h). Scale bar: 10 μm.HQuantification of GFP‐LC3 puncta from 100 MEF cells per experiment cultured in full media (control) or EBSS (starve) for 1 h.IQuantification of WIPI2b puncta in ATG16L1‐complemented HCT116 cells. Puncta from 100 cells were counted per experiment.Data information: Data represent mean ± SEM from three separate experiments. (F) *P < 0.02. (H) ***P < 0.0001, **P < 0.001. (I) ***P < 0.0006, **P≪0.005 (Student's t‐test). Katherine Fletcher et al. EMBO J. 2018;embj.201797840 © as stated in the article, figure or figure legend