Chimerism Analysis in the Pediatric Setting

Slides:

Advertisements

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Advertisements

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Association of Clinical Status of Follicular Lymphoma Patients after Autologous Stem Cell Transplant and Quantitative Assessment of Lymphoma in Blood and.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array Hanneke I. Vos,

Aberrant Levels of miRNAs in Bone Marrow Microenvironment and Peripheral Blood of Myeloma Patients and Disease Progression Weixin Wang, Meghan Corrigan-Cummins,

Bone Marrow Transplant Engraftment Analysis with Loss of an Informative Allele Steven A. Schichman, Pei Lin, Lori J. Gilbrech, Pamela S. Gray, Carla S.

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers Rebecca A. Betensky, Catherine.

Vida Kocbek, Katja Vouk, Nick A. Bersinger, Michael D

Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification Renee Baak-Pablo, Vincent Dezentje, Henk-Jan.

In Silico Proficiency Testing for Clinical Next-Generation Sequencing

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis Jeanne A. Jordan, Mary Beth.

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses Narasimhan Nagan, Nicole E. Faulkner,

Chimerism Monitoring after Allogeneic Hematopoietic Stem Cell Transplantation Using Quantitative Real-Time PCR of Biallelic Insertion/Deletion Polymorphisms

Cystic Fibrosis The Journal of Molecular Diagnostics

Molecular Genetics of Pediatric Soft Tissue Tumors

Whole-Genome Sequencing Identifies Patient-Specific DNA Minimal Residual Disease Markers in Neuroblastoma Esther M. van Wezel, Danny Zwijnenburg, Lily.

A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia Paul A.

Joanna Wang, Chetan Bettegowda The Journal of Molecular Diagnostics

162. Stability of Polymer/Plasmid DNA Complexes In Vitro and In Vivo

Increased Sensitivity of the Roche COBAS AMPLICOR HCV Test, Version 2

Maxim B. Freidin, Neesa Bhudia, Eric Lim, Andrew G

Utilization of Whole-Exome Next-Generation Sequencing Variant Read Frequency for Detection of Lesion-Specific, Somatic Loss of Heterozygosity in a Neurofibromatosis.

Chromosomal Microarray Detection of Constitutional Copy Number Variation Using Saliva DNA Jennifer Reiner, Lisa Karger, Ninette Cohen, Lakshmi Mehta,

Aberrant Levels of miRNAs in Bone Marrow Microenvironment and Peripheral Blood of Myeloma Patients and Disease Progression Weixin Wang, Meghan Corrigan-Cummins,

Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H

Ken B. Waites, Li Xiao, Vanya Paralanov, Rose M. Viscardi, John I

A Lean Laboratory The Journal of Molecular Diagnostics

Challenges in Determining Genotypes for Pharmacogenetics in Allogeneic Hematopoietic Cell Transplant Recipients Loralie J. Langman, Lior Nesher, Dimpy.

Patrick R. Murray The Journal of Molecular Diagnostics

Chronic Myeloid Leukemia

William L. Gerald, M.D., Ph.D, 1954–2008

Bone Marrow Transplant Engraftment Analysis with Loss of an Informative Allele Steven A. Schichman, Pei Lin, Lori J. Gilbrech, Pamela S. Gray, Carla S.

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis Jeanne A. Jordan, Mary Beth.

High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array Hanneke I. Vos,

Screening for Expanded Alleles of the FMR1 Gene in Blood Spots from Newborn Males in a Spanish Population Isabel Fernandez-Carvajal, Paulina Walichiewicz,

Donor Cell Leukemia in Umbilical Cord Blood Transplant Patients

BRAF Mutation Testing in Solid Tumors

Comparison of QIAsymphony Automated and QIAamp Manual DNA Extraction Systems for Measuring Epstein-Barr Virus DNA Load in Whole Blood Using Real-Time.

Multiplex Bead Suspension Array for Screening Neisseria gonorrhoeae Antibiotic Resistance Genetic Determinants in Noncultured Clinical Samples Sergey.

John A. Tynan, Payam Mahboubi, Lesley L

The Molecular Pathology of Primary Immunodeficiencies

Assessment of the Frequency of Allelic Imbalance in Human Tissue Using a Multiplex Polymerase Chain Reaction System Christopher M. Heaphy, William C.

Vida Kocbek, Katja Vouk, Nick A. Bersinger, Michael D

Rare Sequence Variation in the Genome Flanking a Short Tandem Repeat Locus Can Lead to a Question of “Nonmaternity” Anne Deucher, Tsoyu Chiang, Iris.

External Quality Assessment for Detection of Fetal Trisomy 21, 18, and 13 by Massively Parallel Sequencing in Clinical Laboratories Rui Zhang, Hongyun.

Maria Erali, David C. Pattison, Carl T. Wittwer, Cathy A. Petti

Jianbo Song, Danielle Mercer, Xiaofeng Hu, Henry Liu, Marilyn M. Li

Ayalew Tefferi, Pierre Noel, Curtis A. Hanson

Low Incidence of Minor BRAF V600 Mutation-Positive Subclones in Primary and Metastatic Melanoma Determined by Sensitive and Quantitative Real-Time PCR

Avoiding Pitfalls in Molecular Genetic Testing

Danielle C. Smith, Alina Esterhuizen, Jacquie Greenberg

The Distribution of Gene Segments in T-Cell Receptor γ Gene Rearrangements Demonstrates the Need for Multiple Primer Sets Lyle C. Lawnicki, Ronald J.

Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas Qiulu Pan, William Pao, Marc.

Karen Snow-Bailey, Ph.D., 1961–2006

Custom Design of a GeXP Multiplexed Assay Used to Assess Expression Profiles of Inflammatory Gene Targets in Normal Colon, Polyp, and Tumor Tissue Janice.

Extra Alleles in FMR1 Triple-Primed PCR

Journal of Investigative Dermatology

Xiangfeng Cui, Helen Feiner, Honghua Li

Molecular Therapy Volume 21, Pages S247-S248 (May 2013)

Pure White Cell Aplasia (PWCA) Relapsing after Allogeneic BMT and Successfully Treated with Nine DLIs A.M. Marmont, A. Dominietto, MD, F. Gualandi, MD,

Nathan D. Montgomery, Sara R. Selitsky, Nirali M. Patel, D

Improved Detection of the KIT D816V Mutation in Patients with Systemic Mastocytosis Using a Quantitative and Highly Sensitive Real-Time qPCR Assay Thomas.

Multiplex Ligation-Dependent Probe Amplification Identification of Whole Exon and Single Nucleotide Deletions in the CFTR Gene of Hispanic Individuals.

86. A Highly Compact Epitope-Based Marker Suicide Gene for Safer and Easier Adoptive T-Cell Gene Therapy Molecular Therapy Volume 20, Pages S35-S36.

Clinical Experience with the Cervista HPV HR Assay

Angiotensin I-Converting Enzyme

Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards

Optimized Allele-Specific Real-Time PCR Assays for the Detection of Common Mutations in KRAS and BRAF Alois H. Lang, Heinz Drexel, Simone Geller-Rhomberg,

Presentation transcript:

Chimerism Analysis in the Pediatric Setting Susanne Kricke, Lana Mhaldien, Rozendo Fernandes, Charizel Villanueva, Alistair Shaw, Paul Veys, Stuart Adams The Journal of Molecular Diagnostics Volume 20, Issue 3, Pages 381-388 (May 2018) DOI: 10.1016/j.jmoldx.2018.02.003 Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Example data plots from randomly selected samples of whole blood (WB); bone marrow (BM); and cell fractions CD3+, CD15+, and CD19+, comparing quality of peak height and signal strength of the short tandem repeat markers vWA and D3S1358. A and B: vWA marker using the PowerPlex 16 System (A) and the GenePrint 24 System (B) (Promega UK, Southampton, UK). C and D: D3S1358 marker using PowerPlex 16 (C) and GenePrint 24 (D). al, allele; ar, area; sz, size. The Journal of Molecular Diagnostics 2018 20, 381-388DOI: (10.1016/j.jmoldx.2018.02.003) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Results of chimerism analysis of samples of bone marrow; whole blood (including samples from the UK National External Quality Assessment Scheme); and cell fractions CD3+, CD15+, and CD19+, and their corresponding DNA samples, with line of best fit (dotted lines). The Journal of Molecular Diagnostics 2018 20, 381-388DOI: (10.1016/j.jmoldx.2018.02.003) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Results of purity assessment for cell fractions CD3+ and CD19+, and their corresponding DNA samples, with line of best fit (dashed line). The Journal of Molecular Diagnostics 2018 20, 381-388DOI: (10.1016/j.jmoldx.2018.02.003) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions