Ethiopian Mitochondrial DNA Heritage: Tracking Gene Flow Across and Around the Gate of Tears  Toomas Kivisild, Maere Reidla, Ene Metspalu, Alexandra Rosa,

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Ethiopian Mitochondrial DNA Heritage: Tracking Gene Flow Across and Around the Gate of Tears  Toomas Kivisild, Maere Reidla, Ene Metspalu, Alexandra Rosa, Antonio Brehm, Erwan Pennarun, Jüri Parik, Tarekegn Geberhiwot, Esien Usanga, Richard Villems  The American Journal of Human Genetics  Volume 75, Issue 5, Pages 752-770 (November 2004) DOI: 10.1086/425161 Copyright © 2004 The American Society of Human Genetics Terms and Conditions

Figure 1 Map of East Africa and Arabia, with locations of samples collected for the present study. The five cities in Ethiopia where DNA samples were collected are indicated. The main spread zones of the five major Ethiopian linguistic groups that are discussed in the study are shown in blue capital letters. The American Journal of Human Genetics 2004 75, 752-770DOI: (10.1086/425161) Copyright © 2004 The American Society of Human Genetics Terms and Conditions

Figure 2 Median joining network of Ethiopian and Yemeni mtDNA haplotypes. Node sizes are proportional to haplotype frequencies, indicated within nodes for n>1. Haplotypes observed in Ethiopian and Yemeni samples are distinguished by pink and green, respectively. Variable positions (tables A1A2A3A4A5–A6 [online only]) are indicated along links that connect haplotypes. Nucleotide changes are specified only in the case of transversions. A, Network of haplogroups L0–L6. For haplotypes observed in the Yemeni population, matching HVS-I types in samples from northeastern Africa (Krings et al. 1999; Stevanovitch et al. 2004) and Mozambique (Pereira et al. 2001; Salas et al. 2002) are indicated by yellow and blue stars, respectively. B, Network of haplogroups M and N. For haplotypes observed in the Ethiopian population, matching HVS-I types in samples from northeastern Africa (Krings et al. 1999; Stevanovitch et al. 2004) and in the Arabian Peninsula and Iraq (Richards et al. 2000; Al-Zahery et al. 2003) are indicated by yellow and blue stars, respectively. The American Journal of Human Genetics 2004 75, 752-770DOI: (10.1086/425161) Copyright © 2004 The American Society of Human Genetics Terms and Conditions

Figure 3 MDS plot of population differences. The plot is based on FST distances (table A7 [online only]) calculated from haplogroup frequencies; its stress value is 0.065. The American Journal of Human Genetics 2004 75, 752-770DOI: (10.1086/425161) Copyright © 2004 The American Society of Human Genetics Terms and Conditions

Figure 4 Phylogeny of mtDNA complete sequences of haplogroup M1. The tree and the character changes are reconstructed by use of maximum-parsimony criteria. Mutations are numbered relative to revised Cambridge Reference Sequence (rCRS) (Andrews 1999). Nucleotide change is specified only for transversions. Sources: MM1 and MM2 = Maca-Meyer et al. (2001); Herrn 287 = Herrnstadt et al. (2002). Haplogroups M1a and M1b are defined as in figure 2B and tables A1A2A3A4A5–A6 (online only), by polymorphisms identified by Quintana-Murci (1999). Character-state changes at nps 16129 and 16359, shown in parentheses on the external branch of the Herrnstadt et al. (2002) coding-region sequence, are inferred from the complete linkage of the 12346 polymorphism with the derived status at these positions in the present study. The American Journal of Human Genetics 2004 75, 752-770DOI: (10.1086/425161) Copyright © 2004 The American Society of Human Genetics Terms and Conditions