Delineation of HER2 Gene Status in Breast Carcinoma by Silver in Situ Hybridization is Reproducible among Laboratories and Pathologists Antonino Carbone,

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Delineation of HER2 Gene Status in Breast Carcinoma by Silver in Situ Hybridization is Reproducible among Laboratories and Pathologists Antonino Carbone, Gerardo Botti, Annunziata Gloghini, Gianni Simone, Mauro Truini, Maria Pia Curcio, Patrizia Gasparini, Anita Mangia, Tiziana Perin, Sandra Salvi, Adele Testi, Paolo Verderio The Journal of Molecular Diagnostics Volume 10, Issue 6, Pages 527-536 (November 2008) DOI: 10.2353/jmoldx.2008.080052 Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 A and B: This case is equivocal for SISH HER2 gene amplification. Note that chromosome 17 is polysomic. A: In the nuclei there are two or more copies of chromosome 17. The corresponding FISH and CISH results were equivocal (not shown). The Journal of Molecular Diagnostics 2008 10, 527-536DOI: (10.2353/jmoldx.2008.080052) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 The figure shows a typical example of an amplified case as demonstrated by SISH. A: Less than 3 chromosome 17 signals in tumors cells can be seen. B: same case, HER2. Clusters of HER2 signals in tumor cells are recognizable. The Journal of Molecular Diagnostics 2008 10, 527-536DOI: (10.2353/jmoldx.2008.080052) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions