Focal angiogen therapy using intramyocardial delivery of an adenovirus vector coding for vascular endothelial growth factor 1211 Leonard Y Lee, MD, Shailen.

Slides:

Advertisements

Similar presentations

Myocardial functional recovery after fibroblast growth factor 2 gene therapy as assessed by echocardiography and magnetic resonance imaging Keith A Horvath,

Advertisements

Fig. 1. Left coronary artery angiography demonstrates a giant coronary aneurysm (arrowhead) originating from a branch (black arrow) of the left anterior.

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Therapeutic angiogenesis with intramyocardial administration of basic fibroblast growth factor Michio Kawasuji, MD, Hiroshi Nagamine, MD, Masahiro Ikeda,

Therapeutic angiogenesis using autologous bone marrow stromal cells: improved blood flow in a chronic limb ischemia model Abdulaziz Al-Khaldi, MD, Hilal.

Myocardial bridging over the left anterior descending: Myotomy, bypass, or both? Chigozirim N. Ekeke, BS, Stephen Noble, MD, Ernest Mazzaferri, MD, Juan.

Direct epicardial shock wave therapy improves ventricular function and induces angiogenesis in ischemic heart failure Daniel Zimpfer, MD, Seyedhossein.

A novel peroxynitrite decomposer catalyst (FP-15) reduces myocardial infarct size in an in vivo peroxynitrite decomposer and acute ischemia-reperfusion.

Hypercholesterolemia Impairs the Myocardial Angiogenic Response in a Swine Model of Chronic Ischemia: Role of Endostatin and Oxidative Stress Munir Boodhwani,

Surgical treatment of congestive heart failure: evolving options

Coronary artery bypass grafting with the descending branch of thelateral femoral circumflex artery used as an arterial conduit: Is arteriographicevaluation.

An experimental model of saphenous vein-to-coronary artery anastomosis with the St. Jude medical stainless steel connector Hartzell V Schaff, MD, Kenton.

Equivalency of Saphenous Vein and Arterial Composite Grafts: 5-Year Angiography and Midterm Clinical Follow-Up Ho Young Hwang, MD, PhD, Kyung-Hak Lee,

Takotsubo Cardiomyopathy After Coronary Intervention Developed During Hospitalization Jamal Hussain, MD, Nathan Laufer, MD, Suzane Sorrof, MD, Ashish.

Therapeutic angiogenesis in chronically ischemic porcine myocardium: comparative effects of bFGF and VEGF G.Chad Hughes, MD, Shankha S Biswas, MD, Bangliang.

Hiroshi Takami, MD, Taizo Hiraishi, MD, Tohru Kobayashi, MD

Ventricular septal rupture caused by myocardial bridging

Improved Myocardial Perfusion and Thickening After Off-Pump Revascularization: 5- Year Follow-Up Chang Young Kim, MD, Ho Young Hwang, MD, Jin Chul Paeng,

Intravascular imaging and physiologic lesion assessment to define critical coronary stenoses Luis Gruberg, MD, Gary S Mintz, MD, Lowell F Satler, MD,

Michael D. Fridman, MD, Carl Y. Owada, MD, FACC, Richard D

Transmyocardial laser revascularization with excimer laser: clinical results at 1 year Leonard Y Lee, MD, Maureen F O’Hara, RN, Eileen B Finnin, RN, Rory.

Patrick H McNulty, MD, Ian C Gilchrist, MD

Progressive improvement of myocardial perfusion after off-pump revascularization with bilateral internal thoracic arteries: Comparison of early versus.

Surgical Resection of a Giant Coronary Aneurysm

Endogenous myocardial angiogenesis and revascularization using a gastric submucosal patch Marc A Ruel, MD, MPh, Frank W Sellke, MD, Cesario Bianchi,

Calpain inhibition improves collateral-dependent perfusion in a hypercholesterolemic swine model of chronic myocardial ischemia Ashraf A. Sabe, MD, Brittany.

Avoidable Errors in Cardiac Surgery: Anastomosis of the Left Internal Mammary Artery to a Vein Deepak Banerjee, MD, Daniel Fusco, MD, Jeffrey Green,

Aortic root replacement with anomalous origin of the coronary arteries

Travis F. D’Souza, BS, Bennett P. Samuel, MHA, Joseph J

Salvage angiogenesis induced by adenovirus-mediated gene transfer of vascular endothelial growth factor protects against ischemic vascular occlusion

Eduardo A Tovar, MD, Alan Borsari, MD The Annals of Thoracic Surgery

Therapeutic angiogenesis: A comparative study of the angiogenic potential of acidic fibroblast growth factor and heparin Todd K. Rosengart, MD, Kurt.

Transmyocardial laser as an adjunct to minimally invasive CABG for complete myocardial revascularization Naresh Trehan, MD, Yugal Mishra, PhD, Yatin.

J. Trent Magruder, MD, Todd C

Alcohol and the Heart: A Proteomics Analysis of Pericardium and Myocardium in a Swine Model of Myocardial Ischemia Nassrene Y. Elmadhun, MD, Ahmed A.

Improved perfusion and contractile reserve after transmyocardial laser revascularization in a model of hibernating myocardium G.Chad Hughes, MD, Alan.

Subacute limb ischemia induces skeletal muscle injury in genetically susceptible mice independent of vascular density Joseph M. McClung, PhD, Timothy.

Coronary Artery Fistulae Involving Both Right and Left Coronary Arteries Ahmet Bolukcu, MD, Ahmet Can Topcu, MD, Yavuz Sensoz, MD, Ilyas Kayacioglu, MD

Improvement of Coronary Artery Fistula Surgery by Intraoperative Imaging Per Kristian Hol, MD, Odd Geiran, MD, PhD, Kai Andersen, MD, PhD, Karleif Vatne,

Gene therapy with adenovirus-mediated myocardial transfer of vascular endothelial growth factor 121 improves cardiac performance in a pacing model of.

Angiogenic pretreatment to enhance myocardial function after cellular cardiomyoplasty with skeletal myoblasts Mauricio A. Retuerto, BS, James T. Beckmann,

Munir Boodhwani, MD, William E

Efficacy of Therapeutic Angiogenesis by Intramyocardial Injection of pCK-VEGF165 in Pigs Jae-Sung Choi, MD, PhD, Ki-Bong Kim, MD, PhD, Woong Han, DVM,

Up-regulation of vascular endothelial growth factor mrna and angiogenesis after transmyocardial laser revascularization Keith A Horvath, MD, Elaine Chiu,

Volume 12, Issue 4, Pages (October 2005)

Appropriate control of ex vivo gene therapy delivering basic fibroblast growth factor promotes successful and safe development of collateral vessels in.

Volume 19, Issue 4, Pages (April 2011)

Optimal dose and mode of delivery of Na+/H+ exchange-1 inhibitor are critical for reducing postsurgical ischemia-reperfusion injury Joel S Corvera, MD,

Yinsu Zhu, MD, Lijun Tang, MD, PhD, Yi Xu, MD

Ricardo P. J. Budde, PhD, Patricia F. A

Myocardial functional recovery after fibroblast growth factor 2 gene therapy as assessed by echocardiography and magnetic resonance imaging Keith A Horvath,

Comparison of developmental endothelial locus-1 angiogenic factor with vascular endothelial growth factor in a porcine model of cardiac ischemia Murray.

M.Keith Odayan, Hugh S Paterson The Annals of Thoracic Surgery

Transmyocardial laser revascularization: experimental studies in healthy porcine myocardium Georg Lutter, MD, Juergen Martin, MD, Noriyuki Takahashi,

Dipyridamole thallium 201 scintigraphy to detect coronary artery disease before abdominal aortic surgery Bruce S. Cutler, M.D., Jeffrey A. Leppo, M.D.

Peter Pecher, MD, Bernd A Schumacher, MD

Staged Therapeutic Approach in Spontaneous Coronary Dissection

Lethal Postoperative Coronary Artery Spasm

Adam E Saltman, MD, PhD, Walter H Dzik, MD, Sidney Levitsky, MD

Left anterior descending coronary artery dissection after blunt chest trauma: Assessment by multi-detector row computed tomography Tarek Smayra, MD,

An experimental model of chronic myocardial hibernation

Robert R Lazzara, MD, Francis E Kidwell, BS

Abnormal Coronary Artery Connection to the Left Ventricle in a Patient With Coronary Artery Disease Peter Fries, MD, Alexander Massmann, MD, Hermann.

Beating Heart Off-Pump Myocardial Revascularization in an Infant

Therapeutic Angiogenesis With Basic Fibroblast Growth Factor: Technique and Early Results Frank W Sellke, MD, Roger J Laham, MD, Elazer R Edelman, MD,

Amisha J. Patel, MD, Rahul M. Mehta, Divyakant B

Randy M. Stevens, MD, M. Salik Jahania, MD, Robert M

Effects of in vivo myocardial ischemia and reperfusion on interstitial nitric oxide metabolites Randy M Stevens, MD, M.Salik Jahania, MD, Jennifer E.

OPCAB for Acute LAD Dissection Due to Blunt Chest Trauma

Intraoperative Coronary Artery Dissection in Fibromuscular Dysplasia

Presentation transcript:

Focal angiogen therapy using intramyocardial delivery of an adenovirus vector coding for vascular endothelial growth factor 1211 Leonard Y Lee, MD, Shailen R Patel, MD, Neil R Hackett, PhD, Charles A Mack, MD, Dean R Polce, BS, Tarek El-Sawy, BS, Rory Hachamovitch, PhD, Pat Zanzonico, PhD, Timothy A Sanborn, MD, Manish Parikh, PhD, O.Wayne Isom, MD, Ronald G Crystal, MD, Todd K Rosengart, MD The Annals of Thoracic Surgery Volume 69, Issue 1, Pages 14-23 (January 2000) DOI: 10.1016/S0003-4975(99)01102-9

Fig 1 Rat myocardial Ad vector genome levels and VEGF levels over time after intramyocardial administration of the AdGVVEGF121.10 vector. (A) Ad vector genome levels in the apical myocardium, as assessed by TaqMan, relative to the copies of cellular genome. (B) VEGF protein levels in the apical myocardium determined by ELISA expressed as nanograms of VEGF per milligram tissue protein. Values are expressed as the means ± standard error of three animals per data point. The Annals of Thoracic Surgery 2000 69, 14-23DOI: (10.1016/S0003-4975(99)01102-9)

Fig 2 Myocardial Ad vector genome levels in the pig heart 1 h after intramyocardial or intracoronary administration of the AdGVVEGF121.10 vector. The data are presented as the Ad vector genome levels relative to the copies of cellular genome. Values are expressed as the means ± standard error for n = 3 animals in each group. (A) Intramyocardial; (B) intracoronary. The Annals of Thoracic Surgery 2000 69, 14-23DOI: (10.1016/S0003-4975(99)01102-9)

Fig 3 Myocardial VEGF levels in the pig heart 1 day after intramyocardial or intracoronary administration of the AdGVVEGF121.10 vector. Myocardial biopsies were taken for VEGF levels, measured by ELISA, and expressed as nanograms of VEGF protein per milligram tissue protein. Values are expressed as the means ± standard error. (A). Intramyocardial; (B) intracoronary. The Annals of Thoracic Surgery 2000 69, 14-23DOI: (10.1016/S0003-4975(99)01102-9)

Fig 4 Quantitative angiographic assessment of collateral flow to the region of administration of the AdGVVEGF121.10 vector by ex vivo angiography. (A) Shown are representative frames 0, 15, 30, 45, and 60. Small arrows represent circumflex coronary artery, white arrowheads represent the left anterior descending coronary artery, and black arrowhead represents ameroid constrictor. (B) Index of collateral flow. Values are expressed as the means ± standard error for each group. The Annals of Thoracic Surgery 2000 69, 14-23DOI: (10.1016/S0003-4975(99)01102-9)

Fig 5 Representative ex vivo angiograms of pig hearts 28 days after intramyocardial administration of AdGVVEGF121.10 109 pfu or AdNull 109 pfu. (A) AdNull; LAD = left anterior descending artery; (B) AdGVVEGF121.10. The Annals of Thoracic Surgery 2000 69, 14-23DOI: (10.1016/S0003-4975(99)01102-9)

Fig 6 Representative 99mTc-sestamibi SPECT images 28 days after intramyocardial administration of the AdGVVEGF121.10 or AdNull vector to the circumflex territory of the ischemic pig myocardium. Images were obtained at the time of vector administration (day 0) and after 28 days. (A) AdNull; (B and C) AdGVVEGF121.10; (D) semiquantitative 99mTc-sestamibi SPECT imaging regional assessment of circumflex versus septal territory. Values are expressed as the means ± standard error for each group at days 0 and 28 after vector administration. (C = circumflex distribution of the left ventricle; S = septum; ∗ regional ischemia score of zero.) The Annals of Thoracic Surgery 2000 69, 14-23DOI: (10.1016/S0003-4975(99)01102-9)