rSema4A has no direct effect on Treg cells in the in vitro cultures.

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rSema4A has no direct effect on Treg cells in the in vitro cultures. rSema4A has no direct effect on Treg cells in the in vitro cultures. CD4+CD25enriched T cells (Treg cells) were isolated from PBMCs and cultured in vitro with increasing concentrations of rhSema4A with or without rhIL-2. Treg cell cultures with medium alone or medium supplemented rhIL-2 served as negative and positive controls for Sema4A-mediated Treg cell induction, correspondingly. Svetlana P. Chapoval et al. ImmunoHorizons 2019;3:71-87 Copyright © 2019 by The Authors