Intra-individual comparison of human ankle and knee chondrocytes in vitro: relevance for talar cartilage repair C. Candrian, M.D., E. Bonacina, B.Sc.,

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Presentation transcript:

Intra-individual comparison of human ankle and knee chondrocytes in vitro: relevance for talar cartilage repair C. Candrian, M.D., E. Bonacina, B.Sc., J.A. Frueh, B.Sc., D. Vonwil, B.Sc., S. Dickinson, Ph.D., D. Wirz, Ph.D., M. Heberer, M.D., M. Jakob, M.D., I. Martin, Ph.D., A. Barbero, Ph.D. Osteoarthritis and Cartilage Volume 17, Issue 4, Pages 489-496 (April 2009) DOI: 10.1016/j.joca.2008.05.023 Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Histological properties of TC and KC pellets after 2 weeks of culture. (A–B) Safranin O and CII immunohistochemical staining of representative pellets generated by TC and KC from the same donors and cultured for 2 weeks in chondrogenic medium. Bar=100μm. Osteoarthritis and Cartilage 2009 17, 489-496DOI: (10.1016/j.joca.2008.05.023) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Biosynthetic activity of TC and KC pellets after 2 weeks of culture. (A) Sulfate glycosaminoglycan content normalized to the amount of DNA. (B) Real-time reverse transcriptase-polymerase chain reaction analysis of the expression of mRNA for CI and CII. Values are mean±s.e.m. of measurements obtained from 10 independent experiments. Newly synthesized amounts of collagen (C) and proteoglycan (D), measured by the incorporation of, respectively, [3H]proline and [35S]SO4 in pellet maintained in culture for 3 and 14 days. The upper and lower parts of the columns are related, respectively, to the newly synthesized molecules released in the medium or accumulated in the extracellular matrix. Values are mean±s.e.m. of measurements obtained from three independent experiments. *=significantly different from the earlier time point of the same cell source. Osteoarthritis and Cartilage 2009 17, 489-496DOI: (10.1016/j.joca.2008.05.023) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Respose to IL-1β by tissues generated by TC and KC at different stages of maturation. (A) Safranin O stainings of representative pellets generated by TC and KC from the same donors. Bars=100μm. (B) Sulfate glycosaminoglycan content normalized to the amount of DNA in pellets maintained in culture for a total of 14 days where the last 72h with (IL-1) or without control (CTR) IL-1β. Newly synthesized amounts of collagen (C) and proteoglycan (D), measured by the incorporation of, respectively, [3H]proline and [35S]SO4, in pellets maintained in culture 14 days. The upper and lower parts of the columns are related, respectively, to the newly synthesized molecules released in the medium or accumulated in the extracellular matrix. Values are mean±s.e.m. of measurements obtained from three independent experiments. *=significantly different from CTR. Osteoarthritis and Cartilage 2009 17, 489-496DOI: (10.1016/j.joca.2008.05.023) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Histological properties of the engineered cartilaginous tissues generated by talar chondrocytes and knee chondrocytes after 4 weeks of static culture. (A–B) Safranin O and CII immunohistochemical stainings of representative ECT or ECK. Bar=100μm. Osteoarthritis and Cartilage 2009 17, 489-496DOI: (10.1016/j.joca.2008.05.023) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Biosynthetic activity and biomechanical properties of engineered cartilage generated by talar chondrocytes (ECT) and engineered cartilage generated by knee chondrocytes (ECK) after 2 and 4 weeks of static culture. (A) Amounts of GAG, CI and CII accumulated, expressed as a percentage of tissue dry weight. (B) Equilibrium modulus and dynamic pulsatile modulus of ECT and ECK constructs. The plotted line indicates the biomechanical properties of the cell-free Hyaff-11 scaffold. Values are mean±s.e.m. of measurements obtained from 10 independent experiments. *=significantly different from the earlier time point of the same cell source; ○=significantly different from ECT. Osteoarthritis and Cartilage 2009 17, 489-496DOI: (10.1016/j.joca.2008.05.023) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions