Raymond R. Tubbs, James Pettay, Pat Roche, Mark H

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Concomitant Oncoprotein Detection with Fluorescence in Situ Hybridization (CODFISH) Raymond R. Tubbs, James Pettay, Pat Roche, Mark H. Stoler, Robert Jenkins, Jon Myles, Thomas Grogan The Journal of Molecular Diagnostics Volume 2, Issue 2, Pages 78-83 (May 2000) DOI: 10.1016/S1525-1578(10)60620-4 Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Fluorescence confocal microscopy photomicrograph, cell line known to have normal Her-2/neu gene copy and absence of oncoprotein expression. One, three, or four Her-2/neu gene copies are identified in each nucleus (green). No surface oncoprotein is present. The Journal of Molecular Diagnostics 2000 2, 78-83DOI: (10.1016/S1525-1578(10)60620-4) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Fluorescence confocal microscopy photomicrograph, BT-474 cell line. Numerous Her-2/neu gene copies (green) are simultaneously visualized along with abundant surface membrane and some cytoplasmic oncoprotein expression (red). The Journal of Molecular Diagnostics 2000 2, 78-83DOI: (10.1016/S1525-1578(10)60620-4) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Fluorescence confocal microscopy photomicrograph. Infiltrating ductal carcinoma of breast demonstrating normal Her-2/neu gene copy and absence of oncoprotein expression. The Journal of Molecular Diagnostics 2000 2, 78-83DOI: (10.1016/S1525-1578(10)60620-4) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Fluorescence confocal microscopy photomicrograph. Abundant Her-2/neu gene copy (green) and oncoprotein (red) overexpression are simultaneously visualized. Infiltrating ductal carcinoma of breast. The Journal of Molecular Diagnostics 2000 2, 78-83DOI: (10.1016/S1525-1578(10)60620-4) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions