Cell allocation and cell death in blastocysts from nonhuman primates generated during in vitro fertilization and intracytoplasmic sperm injection Evelyn.

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Cell allocation and cell death in blastocysts from nonhuman primates generated during in vitro fertilization and intracytoplasmic sperm injection Evelyn Neuber, Ph.D., Anthony W.S Chan, Ph.D., Tanja Dominko, Ph.D., Calvin Simerly, Ph.D., Gerald Schatten, Ph.D. Fertility and Sterility Volume 77, Issue 5, Pages 1083-1085 (May 2002) DOI: 10.1016/S0015-0282(02)02996-5

FIGURE 1 Three-dimensional reconstruction of a confocal imaged IVF generated rhesus blastocyst labeled with Hoechst 33342 (total DNA, blue) and TUNEL assay (apoptotic nuclei, green). (A), The complete 3-D reconstruction provided the total cell number, (B), Focusing on the middle slices allowed discrimination between the ICM and the TE cells (no TUNEL shown). Bar = 10 μm. Neuber. Blastocysts generated from nonhuman primates. Fertil Steril 2002. Fertility and Sterility 2002 77, 1083-1085DOI: (10.1016/S0015-0282(02)02996-5)