Vitamin C and vitamin E supplementation reduce oxidative stress–induced embryo toxicity and improve the blastocyst development rate Xia Wang, M.D., Ph.D.,

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Vitamin C and vitamin E supplementation reduce oxidative stress–induced embryo toxicity and improve the blastocyst development rate Xia Wang, M.D., Ph.D., Tommaso Falcone, M.D., Marjan Attaran, M.D., Jeffrey M Goldberg, M.D., Ashok Agarwal, Ph.D., Rakesh K Sharma, Ph.D. Fertility and Sterility Volume 78, Issue 6, Pages 1272-1277 (December 2002) DOI: 10.1016/S0015-0282(02)04236-X

FIGURE 1 The linear response (mean ± SD) of log (ROS + 1) values versus activated leukocyte concentrations. Reactive oxygen species (ROS) response was determined in triplicate by using activated leukocyte concentrations ranging from 0.25 to 4.0 × 106 cells/mL. Wang. ROS-induced embryotoxicity and antioxidants.Fertil Steril 2002. Fertility and Sterility 2002 78, 1272-1277DOI: (10.1016/S0015-0282(02)04236-X)

FIGURE 2 Effect of antioxidant supplementation on mouse embryo development, expressed as the blastocyst development rate (BDR). (A), Effect of vitamin C. The control group consisted of embryos cultured in human tubal fluid medium. (B), Effect of vitamin E. Wang. ROS-induced embryotoxicity and antioxidants.Fertil Steril 2002. Fertility and Sterility 2002 78, 1272-1277DOI: (10.1016/S0015-0282(02)04236-X)

FIGURE 3 Comparison of ROS scavenging activity of the antioxidants vitamin C and vitamin E on development of mouse embryos, expressed as the mean overall blastocyst development rate (BDR). Control = embryos cultured in human tubal fluid medium only; PMA = embryos cultured in 12-phorbol 13-myristate acetate (PMA)–activated leukocyte supernatant; PMA + vitamin C = embryos cultured in PMA-activated leukocyte supernatant and vitamin C (50 μM); PMA + vitamin E = embryos cultured in PMA-activated leukocyte supernatant and vitamin E (400 mg). Reactions were done for 3 hours and 6 hours. At 6 hours, vitamin C significantly reversed the embryotoxicity induced by PMA–activated leukocyte supernatant. Wang. ROS-induced embryotoxicity and antioxidants.Fertil Steril 2002. Fertility and Sterility 2002 78, 1272-1277DOI: (10.1016/S0015-0282(02)04236-X)