Volume 10, Issue 5, Pages (March 2000)

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Volume 10, Issue 5, Pages 269-272 (March 2000) Protein phosphatase 1β is required for the maintenance of muscle attachments S. Raghavan, I. Williams, H. Aslam, D. Thomas, B. Szöőr, G. Morgan, S. Gross, J. Turner, J. Fernandes, K. VijayRaghavan, L. Alphey Current Biology Volume 10, Issue 5, Pages 269-272 (March 2000) DOI: 10.1016/S0960-9822(00)00364-X

Figure 1 Molecular characterisation of PP1β9C. (a) Gene structure of PP1β9C. Sites for the restriction enzymes BglII (B), EcoRI (E) and XhoI (X) are marked. DS03519 and DS06782 are P1 clones from the Berkeley Drosophila genome project, 121A3 is a cosmid from the European Drosophila Genome Project. (b) Comparison of the intron/exon structure of human and fly PP1β within the 981 nt coding region. The nucleotide positions of the four fly introns are shown; three of them correspond exactly with introns in the human homologue. (c) Western blot of pupal proteins probed for PP1β. Similar levels of PP1β protein are present in wild-type and mutant pupae. (d–g) Sequences of mutated regions in flw1 and flw6, with traces from an automatic sequencer below. (d,e) At the codon corresponding to Val284 (GTG) in (d) the wild type, (e) flw1 has GCG, encoding Ala. (f,g) At the codon corresponding to Tyr133 (TAC) in (f) the wild type, (g) flw6 has TTC, encoding Phe. Current Biology 2000 10, 269-272DOI: (10.1016/S0960-9822(00)00364-X)

Figure 2 Muscle defects in flw6 males. (a–d) IFMs; anterior is left, dorsal is uppermost. (a) Diagram of thoracic musculature (reproduced from [21] with permission). Black, DLMs; grey, DVMs. (b–d) Polarised light micrographs showing thoracic muscles of (b) wild-type, (c) flw6/Y, and (d) flw6/Y; P[hsp26-PP1β9C]/+ males. The latter express PP1β9C from the hsp26 promoter. IFMs are completely absent in (c) and as wild type in (d). (e,f) Larval body wall muscles (stained blue in a histochemical reaction for β-galactosidase expressed by a myosin heavy chain–LacZ reporter gene) of (e) wild-type and (f) flw6/Y males. Arrows point to poorly attached, remnant muscles in the mutant (f) and to the corresponding region in (e). Asterisks mark a region devoid of muscles in the mutant (f) and the corresponding region in (e). Anterior is to the top and the dorsal midline is right of centre. Current Biology 2000 10, 269-272DOI: (10.1016/S0960-9822(00)00364-X)

Figure 3 Wing defects in flw mutants. (a) Wild type; (b)flw1/Y; (c)flw6/Y; (d) flw6/Y ; P[hsp26-PP1β9C]/+. Wings of flw6/Y flies are severely crumpled and/or blistered; this defect is completely rescued by the PP1β9C expression construct. Current Biology 2000 10, 269-272DOI: (10.1016/S0960-9822(00)00364-X)