Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing Susanne Heiss, Rudolf Valenta

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Presentation transcript:

Component-Resolved Diagnosis (CRD) of Type I Allergy with Recombinant Grass and Tree Pollen Allergens by Skin Testing Susanne Heiss, Rudolf Valenta Journal of Investigative Dermatology Volume 113, Issue 5, Pages 830-837 (November 1999) DOI: 10.1046/j.1523-1747.1999.00796.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Coomassie blue-stained gel containing pollen extracts and purified recombinant pollen allergens. Birch pollen (birch) and timothy grass pollen extracts as well as recombinant birch (rBet v 1, rBet v 2) and timothy grass pollen allergens (rPhl p 1, rPhl p 2, rPhl p 5) were separated by SDS-PAGE and stained with Coomassie blue. Molecular weights (kDa) are indicated on the left margin. Journal of Investigative Dermatology 1999 113, 830-837DOI: (10.1046/j.1523-1747.1999.00796.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Serum IgE-reactivity to nitrocellulose blotted natural timothy grass pollen extract. Sera from grass pollen allergic patients (lanes 1–52, 58, 61), from an allergic patient without grass pollen allergy (lane 53), and from nonallergic individuals (lanes 54–57, 59–60, 62–64) were probed with nitrocellulose blotted timothy grass pollen extract. Bound IgE was detected with 125I-labeled anti-human IgE antibodies and visualized by autoradiography. The molecular weights (kDa) are displayed on the left side. Journal of Investigative Dermatology 1999 113, 830-837DOI: (10.1046/j.1523-1747.1999.00796.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Serum IgE-reactivity to nitrocellulose blotted natural birch pollen extract. Sera from birch pollen allergic patients (lanes 1, 6, 7, 9, 13, 14, 16–22, 25, 27, 29, 31–34, 36–38, 41–45, 47, 49–53, 58, 61), from atopic individuals without birch pollen allergy (lanes 2–5, 8, 10–12, 15, 23, 24, 26, 28, 30, 35, 39, 40, 46, 48), and from nonallergic individuals (lanes 54–57, 59–60, 62–64) were probed with nitrocellulose blotted birch pollen extract. Bound IgE was detected with 125I-labeled anti-human IgE antibodies and visualized by autoradiography. The molecular weights (kDa) are displayed on the left side. Journal of Investigative Dermatology 1999 113, 830-837DOI: (10.1046/j.1523-1747.1999.00796.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Skin prick activity of recombinant pollen allergens in representative patients. The wheal reactions of five representative patients (a, #19; b, #5; c, #28; d, #11; e, #21) were documented by photography. The dose of the allergens as well as the application order of allergens, histamine, and sodium chloride solution are displayed in the insert. Journal of Investigative Dermatology 1999 113, 830-837DOI: (10.1046/j.1523-1747.1999.00796.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions