Improved profile of bad phosphorylation and caspase 3 activation after blood versus crystalloid cardioplegia Jun Feng, MD, PhD, Cesario Bianchi, MD,

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Improved profile of bad phosphorylation and caspase 3 activation after blood versus crystalloid cardioplegia Jun Feng, MD, PhD, Cesario Bianchi, MD, PhD, Jianyi Li, MD, Frank W Sellke, MD The Annals of Thoracic Surgery Volume 77, Issue 4, Pages 1384-1389 (April 2004) DOI: 10.1016/j.athoracsur.2003.09.053

Fig 1 Response to SNP in vitro of rabbit coronary microvessels from control hearts (n = 6, •) and hearts after 30 minutes of ischemic arrest using CCP (n = 6, ▴) or BCP (n = 6, ■) followed by 30 minutes of reperfusion. (BCP = blood cardioplegia; CCP = crystalloid cardioplegia; SNP = sodium nitroprusside.) The Annals of Thoracic Surgery 2004 77, 1384-1389DOI: (10.1016/j.athoracsur.2003.09.053)

Fig 2 Response to ADP in vitro of rabbit coronary microvessels from control hearts (n = 6, •) and hearts after 30 minutes of ischemic arrest using CCP (n = 6, ■) or BCP (n = 6, ▴) followed by 30 minutes of reperfusion. *p < 0.05 BCP versus CCP. (ADP = adenosine 5′-diphosphate; BCP = blood cardioplegia; CCP = crystalloid cardioplegia.) The Annals of Thoracic Surgery 2004 77, 1384-1389DOI: (10.1016/j.athoracsur.2003.09.053)

Fig 3 Caspase 3 cleavage: protein was obtained from control hearts and hearts after 30 minutes of ischemic arrest using CCP or BCP followed by 30 minutes of reperfusion. (A) Representative blots showing proform (32 kda) and p17 subunit of caspase 3 protein levels in different groups. (B) Graph showing the fold-increase of p17 subunit of caspase 3 protein levels in different groups; n = 6 for each group *p < 0.05, **p < 0.01 compared with control; †p < 0.05 compared with CCP. (BCP = blood cardioplegia; CCP = crystalloid cardioplegia.) The Annals of Thoracic Surgery 2004 77, 1384-1389DOI: (10.1016/j.athoracsur.2003.09.053)

Fig 4 Western blots showing total Bcl-2 (A) and Bax (B) protein levels from control hearts and hearts after 30 minutes of ischemic arrest using CCP or BCP, followed by 30 minutes of reperfusion. Graph showing the fold-increase in Bcl-2 (A) and Bax (B) protein expression in different groups; n = 6 for each group. (BCP = blood cardioplegia; CCP = crystalloid cardioplegia.) The Annals of Thoracic Surgery 2004 77, 1384-1389DOI: (10.1016/j.athoracsur.2003.09.053)

Fig 5 Phosphorylation of Ser112 of the pro-apoptotic protein Bad from control hearts and hearts after 30 minutes of ischemic arrest using CCP or BCP followed by 30 minutes of reperfusion. (A) Western blots showing total and phosphorylated Bad protein levels. (B) Graph showing the fold-increase in Bad phosphorylation in different groups; n = 6 for each group, *p < 0.05, **p < 0.01 compared with control, †p < 0.05 compared with CCP. (BCP = blood cardioplegia; CCP = crystalloid cardioplegia.) The Annals of Thoracic Surgery 2004 77, 1384-1389DOI: (10.1016/j.athoracsur.2003.09.053)