Separation of human immunodeficiency virus type 1 from motile sperm by the double tube gradient method versus other methods  Joseph A Politch, Ph.D.,

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Separation of human immunodeficiency virus type 1 from motile sperm by the double tube gradient method versus other methods  Joseph A Politch, Ph.D., Chong Xu, M.D., Lynne Tucker, M.Sc., Deborah J Anderson, Ph.D.  Fertility and Sterility  Volume 81, Issue 2, Pages 440-447 (February 2004) DOI: 10.1016/j.fertnstert.2003.06.028

Figure 1 Schematic representations of sperm-processing techniques. Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004. Fertility and Sterility 2004 81, 440-447DOI: (10.1016/j.fertnstert.2003.06.028)

Figure 2 Comparison of HIV-1 RNA in the motile sperm fractions from single tube gradient (n = 3), swim-up (n = 3), and double tube gradient (n = 3) methods using ISolate sperm separation medium. Data are represented as mean ± standard error HIV-1 RNA copy number per motile sperm fraction. Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004. Fertility and Sterility 2004 81, 440-447DOI: (10.1016/j.fertnstert.2003.06.028)

Figure 3 Comparison of HIV-1 RNA in the motile sperm fractions from single tube gradient (n = 6), gradient/swim-up (n = 6), and double tube gradient (n = 6) methods using ISolate sperm separation medium. Data are represented as mean ± standard error HIV-1 RNA copy number per motile sperm fraction. Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004. Fertility and Sterility 2004 81, 440-447DOI: (10.1016/j.fertnstert.2003.06.028)

Figure 4 Comparison of sperm yield in the motile sperm fractions from single tube gradient , double tube gradient , and gradient/swim-up methods using ISolate and Percoll sperm separation media. Data are represented as mean ± standard error percent total motile sperm of initial sample. No statistically significant differences in sperm yield were found between ISolate and Percoll. Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004. Fertility and Sterility 2004 81, 440-447DOI: (10.1016/j.fertnstert.2003.06.028)