Characterization of mature vs aged rabbit articular cartilage: analysis of cell density, apoptosis-related gene expression and mechanisms controlling.

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Presentation transcript:

Characterization of mature vs aged rabbit articular cartilage: analysis of cell density, apoptosis-related gene expression and mechanisms controlling chondrocyte apoptosis R. Todd Allen, M.D., Ph.D., Catherine M. Robertson, M.D., Frederick L. Harwood, B.Sc., Takahisa Sasho, M.D., Ph.D., Seth K. Williams, M.D., Adam C. Pomerleau, B.A., David Amiel, Ph.D. Osteoarthritis and Cartilage Volume 12, Issue 11, Pages 917-923 (November 2004) DOI: 10.1016/j.joca.2004.08.003 Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Example of grade I articular cartilage on a rabbit femoral condyle and tibial plateau. Top panels are photographs of untreated joints and bottom panels are after india ink staining. M: medial; L: lateral. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 2 Example of confocal microscopy following staining for viable chondrocytes in the superficial, mid and deep zones of mature and aged rabbit tibial plateau articular cartilage. Note: brightly white-stained chondrocytes indicate viable cells. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 3 VCD in the superficial, mid and deep zones of mature and aged (n=6, each) rabbit tibial plateau and femoral condyle articular cartilage: *=P<0.05, aged vs mature. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 4 Agarose gel electrophoresis following RT-PCR of RNA extracted from representative samples of mature and aged rabbit articular cartilage. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 5 Relative expression of apoptosis-related genes in mature and aged rabbit articular cartilage as determined by NIH Image analysis following RT-PCR and agarose gel electrophoresis: *=P<0.05, aged vs mature; n=6, each. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 6 Dose-dependent apoptotic response of mature rabbit articular chondrocytes to nitroprusside (SNP); n=8. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions

Fig. 7 The effect of PI3 kinase inhibitor and MEK-MAPK kinase inhibitors on IGF-1 inhibition of SNP-induced apoptosis: *=P<0.05, n=8. Note that the PI3 kinase inhibitor reversed IGF-1 inhibition but MEK-MAPK inhibitor had no effect. Osteoarthritis and Cartilage 2004 12, 917-923DOI: (10.1016/j.joca.2004.08.003) Copyright © 2004 OsteoArthritis Research Society International Terms and Conditions