Differences in morphology, growth rate, and protein synthesis between cultured arterial and venous endothelial cells Willis H. Wagner, MD, Renee M. Henderson,

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Presentation transcript:

Differences in morphology, growth rate, and protein synthesis between cultured arterial and venous endothelial cells Willis H. Wagner, MD, Renee M. Henderson, BS, Heraline E. Hicks, PhD, Albert J. Banes, PhD, George Johnson, MD Journal of Vascular Surgery Volume 8, Issue 4, Pages 509-519 (October 1988) DOI: 10.1016/0741-5214(88)90119-X Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Representative cell sizing of individual aortic (A) and vena caval (B) endothelial cell samples. Mean cell size from each sample was derived from these curves. Note that size is given as the diameter of spherical cells in suspension (see “Material and Methods”). Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Phase-contrast micrographs of confluent, first passage, bovine aortic (A) and vena caval (B) endothelial cells grown on polystyrene culture dishes (original magnification × 170). Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Scanning electron micrographs of fifth-passage aortic (A) and vena caval (B) endothelial cells grown on glass slides (original magnification × 1500 A and B). The cell in the center of the venous field (B) demonstrates the numerous fine intercellular processes of the venous endothelial cells that were not found with the aortic counterparts. The surrounding confluent cells (B) are representative of the relatively larger venous cells (|—| = 10 μm). Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Growth curve of aortic (AEC) and vena caval (VEC) endothelial cells on polystyrene (n = 6 for both AEC and VEC for each point on the curve). The initial plating density was 3.0 × 104 cells per well of six well plates. *, p < 0.05 by t test. Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Daily cell sizing of aortic (AEC) and vena caval (VEC) endothelial cells during the growth curve experiment (n = 6 for both AEC and VEC for each point on the curve). *, p < 0.05 by t test. Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 6 Autoradiographs of 35S-methionine-labeled proteins from bovine aortic (A) and vena caval (B) endothelial cells separated by two-dimensional gel electrophoresis (20 cm × 0.8 mm pH 3-10 ampholine focusing gel and 20 × 20 cm 12.5% SDS slab gel). An average of 943 aortic and 807 vena caval proteins were separated by this technique. The PDQUEST computer analysis program was used to quantitate the densities of the protein spots and to compare the patterns of the AEC to those of the VEC. Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 7 Protein spot differences between aortic and vena caval endothelial cells. Differences are significant to p < 0.05.% Total, Percentage of 257 protein spots. Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 8 A, Scatter plot for all protein spots detected in autoradiographs for one aortic endothelial cell sample vs another (AEC vs AEC). The correlation coefficient of 0.91 indicates a great degree of homology between samples within a group (a value of 1 would be a perfect correlation indicating complete homology between samples). Similar correlation coefficients were obtained for the venous cell samples. B, Scatter plot for all protein spots detected for an aortic endothelial cell sample versus a vena caval endothelial cell sample (AEC vs VEC). The correlation coefficient 0.73 indicates that the samples were substantially different from each other. In fact, approximately 25% of the proteins detected between the groups were changed in amount or present in one group but absent in the other. Journal of Vascular Surgery 1988 8, 509-519DOI: (10.1016/0741-5214(88)90119-X) Copyright © 1988 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions