Ion Exchange worksheet Due at the beginning of lecture on Thursday, April 7th, 2011

Ion exchange worksheet due April 7th Please hand in a neatly presented (typed): 1. Table with absorbance and corresponding concentration values 2. A graph of your GFP standard curve, be sure to label the axis 3. The concentration of your purified GFP (mg/ml) 4. The total amount of GFP purified (mg) 5. The volume (ml) of your purified GFP 6. Percent recovery: assuming your initial sample mixture (0.5ml) contained 1.0mg/ml GFP Note: If you diluted your purified GFP remember to use the dilution factor in your calculations