Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in.

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Increased matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-1 secretion but unaffected invasiveness of endometrial stromal cells in adenomyosis Jehn-Hsiahn Yang, M.D., Ming-Yih Wu, M.D., Mei-Jou Chen, M.D., Shee-Uan Chen, M.D., Yu-Shih Yang, M.D., Ph.D., Hong-Nerng Ho, M.D. Fertility and Sterility Volume 91, Issue 5, Pages 2193-2198 (May 2009) DOI: 10.1016/j.fertnstert.2008.05.090 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Immunohistochemical staining of MMP-2, MMP-9, TIMP-1 and TIMP-2 in ectopic (A, B, C, D) and eutopic endometrium (E, F, G, H) of women with adenomyosis. Immunoreactive stromal cells (arrows) are visible in both ectopic and eutopic endometrium. The immunoreactive stromal cells are more prominent in ectopic endometrium than those in eutopic endometrium. Positive staining for endometrial glands is also found. Staining patterns vary from case to case. Fertility and Sterility 2009 91, 2193-2198DOI: (10.1016/j.fertnstert.2008.05.090) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Immunocytochemical staining of MMP-2, MMP-9, TIMP-1, TIMP-2, and negative controls in ESCs from women with adenomyosis (A, B, C, D, E) and without adenomyosis (F, G, H, I, J). The immunoreactive ESCs stained with immunocytochemistry are more prominent than those in the eutopic endometrium stained with immunohistochemistry. Scale bar, 30 μm. Fertility and Sterility 2009 91, 2193-2198DOI: (10.1016/j.fertnstert.2008.05.090) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 The Matrigel invasion assay of endometrial stromal cells (ESCs) in women with adenomyosis (black bars, n = 21) and without adenomyosis (white bars, n = 25). All values are expressed as mean ± SD. ESCs were incubated alone, with IL-6 (10 ng/mL), anti-IL-6 (0.5 μg/mL), or with GM6001 (10 μM) for 24 hours. The abilities of cell invasion, using ESCs cultured alone in the control group as the bench mark, were all similar between the two groups. Fertility and Sterility 2009 91, 2193-2198DOI: (10.1016/j.fertnstert.2008.05.090) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 The MMP-2, MMP-9, TIMP-1, and TIMP-2 concentrations in the supernatants of endometrial stromal cells (ESCs) in women with adenomyosis (●, n = 21) and without adenomyosis (○, n = 25). The median value is indicated by (—) in each column. ∗P=.023 by Mann-Whitney test. ∗∗P=.007 by Mann-Whitney test. Fertility and Sterility 2009 91, 2193-2198DOI: (10.1016/j.fertnstert.2008.05.090) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions