Electroacupuncture decreases the progression of ovarian hyperstimulation syndrome in a rat model Li Chen, Hai-Xiang Sun, You-Bing Xia, Liu-Cai Sui, Ji.

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Electroacupuncture decreases the progression of ovarian hyperstimulation syndrome in a rat model Li Chen, Hai-Xiang Sun, You-Bing Xia, Liu-Cai Sui, Ji Zhou, Xuan Huang, Jing-Wei Zhou, Yi-Dan Shao, Tao Shen, Qin Sun, Yuan-Jiao Liang, Bing Yao Reproductive BioMedicine Online Volume 32, Issue 5, Pages 538-544 (May 2016) DOI: 10.1016/j.rbmo.2016.02.006 Copyright © 2016 The Authors Terms and Conditions

Figure 1 Effects of electroacupuncture treatment on ovary weight and capillary permeability in rats induced to achieve ovarian hyperstimulation syndrome. (A) Comparison of rat ovary weight between groups; (B) comparison of Evans Blue concentration in peritoneal irrigated fluid; (C) comparison of Evans Blue concentration in the ovarian extract. Values are expressed as mean ± SEM. *P < 0.05, **P < 0.01. EB, Evans Blue dye. EAO, electroacupuncture plus OHSS; OHSS, ovarian hyperstimulation syndrome; PEAO, electroacupuncture pretreatment plus OHSS. Reproductive BioMedicine Online 2016 32, 538-544DOI: (10.1016/j.rbmo.2016.02.006) Copyright © 2016 The Authors Terms and Conditions

Figure 2 Effects of electroacupuncture on serum levels of (A) oestradiol; (B) prolactin (B); (C) progesterone and (D) testosterone in rats induced to achieve ovarian hypserstimulation syndrome. The level of four hormones was determined by chemiluminescent immunoassay. Values are represented as mean ± SEM. *P < 0.05, **P < 0.01. EAO, electroacupuncture plus OHSS; OHSS, ovarian hyperstimulation syndrome; PEAO, electroacupuncture pretreatment plus OHSS. Reproductive BioMedicine Online 2016 32, 538-544DOI: (10.1016/j.rbmo.2016.02.006) Copyright © 2016 The Authors Terms and Conditions

Figure 3 Effect of electroacupuncture treatment on the serum levels of (A) MCP-1; (B) TNF-α and (C) ovarian IL-6 in rats induced to achieve ovarian hypserstimulation syndrome. The levels of MCP-1 and TNF-a in serum and IL-6 in ovarian extract were analysed by enzyme-linked immunosorbent assay. Values are represented as mean ± SEM. * P < 0.05; **P < 0.01. EAO, electroacupuncture plus OHSS; IL-6, interleukin-6; MCP-1, monocyte chemoactic protein 1; OHSS, ovarian hyperstimulation syndrome; PEAO, electroacupuncture pretreatment plus OHSS; TNF-α, tumour necrosis factor alpha. Reproductive BioMedicine Online 2016 32, 538-544DOI: (10.1016/j.rbmo.2016.02.006) Copyright © 2016 The Authors Terms and Conditions

Figure 4 Effect of electroacupuncture treatment on vascular endothelial growth factor expression in the ovary. vascular endothelial growth factor concentration was determined by enzyme-linked immunosorbent assay. Values are represented as mean ± SEM. *P < 0.05, **P < 0.01. EAO, electroacupuncture plus OHSS; OHSS, ovarian hyperstimulation syndrome; PEAO, electroacupuncture pretreatment plus OHSS; VEGF, vascular endothelial growth factor. Reproductive BioMedicine Online 2016 32, 538-544DOI: (10.1016/j.rbmo.2016.02.006) Copyright © 2016 The Authors Terms and Conditions