Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level F. Araujo, T. Slifer, S.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

Pyrazinamide resistance in multidrug-resistant Mycobacterium tuberculosis isolates in Japan H. Ando, S. Mitarai, Y. Kondo, T. Suetake, J.-I. Sekiguchi,

Circulating levels of sTNFR and discrepancy between cytotoxicity and immunoreactivity of TNF-α in patients with visceral leishmaniasis I.M. Medeiros,

Volume 118, Issue 4, Pages (April 2000)

Cerebrospinal fluid cytokines in enterovirus 71 brain stem encephalitis and echovirus meningitis infections of varying severity S.-M. Wang, H.-Y. Lei,

Gut bacterial microbiota and obesity

Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Characterization of TNF-α– and IL-17A–Mediated Synergistic Induction of DEFB4 Gene Expression in Human Keratinocytes through IκBζ Claus Johansen, Trine.

Migrant health—a cause for concern?

Clinical characteristics of Haemophilus influenzae meningitis in Denmark in the post- vaccination era T.I. Pedersen, M. Howitz, C. Østergaard Clinical.

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

Toll-like receptors in Borrelia burgdorferi-induced inflammation

J.-P. Van geertruyden Clinical Microbiology and Infection

Y. Higashimoto, M. Ohata, Y. Yamagata, T. Iwata, M. Masuda, T

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes N. Chabane, M.Sc.,

E. Cambau, M. Drancourt Clinical Microbiology and Infection

Louis Pasteur, from crystals of life to vaccination

How to evaluate and predict the ecologic impact of antibiotics: the pharmaceutical industry view from research and development R. Bax Clinical Microbiology.

Yiping Wang, Yuet-Ching Tay, David C.H. Harris Kidney International

Chlamydia trachomatis infections in heterosexuals attending sexually transmitted disease clinics in Slovenia D. Kese, M. Maticic, M. Potocnik Clinical.

Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor- kappa B pathway in neuroglioma cells L. Zhang, J. Zhang, L. Yang,

Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide F.G. Araujo, T.L. Slifer, J.S. Remington Clinical.

Assessment of nephrotoxicity in patients receiving amphotericin B lipid complex: a pharmacosurveillance study in Spain J.-M. Aguado, C. Lumbreras, D.

Volume 116, Issue 6, Pages (June 1999)

Vector control: a cornerstone in the malaria elimination campaign

Volume 68, Issue 1, Pages (July 2005)

Breast milk causing neonatal sepsis and death

L. Y. A. Chai, R. Naesens, A. L. Khoo, M. V. Abeele, K

Training for the infectious diseases speciality in Norway

Levofloxacin in the treatment of ventilator-associated pneumonia

G. Pappas Clinical Microbiology and Infection

Similar inflammatory response in human whole blood to live Streptococcus pneumoniae of different serotypes P. Kragsbjerg, M. Jurstrand, H. Fredlund

R. Bitterman, M. Paul, D. Polak, Y. Geffen

Metagenomics and probiotics

Inverse Regulation of the Nuclear Factor-κB Binding to the p53 and Interleukin-8 κB Response Elements in Lesional Psoriatic Skin Claus Johansen, Esben.

Volume 119, Issue 1, Pages (July 2000)

Laboratory diagnosis of Clostridium difficile disease

The practice of travel medicine in Europe

Porins and lipopolysaccharide from Salmonella typhimurium regulate the expression of CD80 and CD86 molecules on B cells and macrophages but not CD28 and.

Ketoconazole Suppresses Prostaglandin E2-Induced Cyclooxygenase-2 Expression in Human Epidermoid Carcinoma A-431 Cells Naoko Kanda, Dr., Shinichi Watanabe

Abstracts cont. Clinical Microbiology and Infection

17β-estradiol Inhibits the Production of RANTES in Human Keratinocytes

A.P. Underwood, J. Green Clinical Microbiology and Infection

Andrew M. Middleton, Maureen V. Chadwick, Harold Gaya

Statin use and clinical outcomes among pneumonia patients

STAT proteins mediate angiotensin II–induced production of TIMP-1 in human proximal tubular epithelial cells Xiangmei Chen, Jianzhong Wang, Feng Zhou,

S. Alexiou-Daniel, A. Stylianakis, A. Papoutsi, I. Zorbas, A. Papa, A

K. Kaier, N.T. Mutters, U. Frank Clinical Microbiology and Infection

The atypical pneumonias: clinical diagnosis and importance

Facilitating learning and change in physicians: Implications for a system of continuing medical education in Europe Robert D. Fox Clinical Microbiology.

Modelling during an emergency: the 2009 H1N1 influenza pandemic

New oral cephalosporins in pediatric community-acquired infections

Dysregulated interleukin-8 secretion and NF-κB activity in human cystic fibrosis nasal epithelial cells Salvatore Carrabino, Daniela Carpani, Alessandra.

Dimethylfumarate Specifically Inhibits the Mitogen and Stress-Activated Kinases 1 and 2 (MSK1/2): Possible Role for its Anti-Psoriatic Effect Borbala.

John W. Haycock, Mark Wagner, Sheila Mac Neil

Blazej Zbytek, Andrzej T. Slominski

J.L. Balcázar Clinical Microbiology and Infection

G.C. Schito Clinical Microbiology and Infection

Angiotensin III increases MCP-1 and activates NF-кB and AP-1 in cultured mesangial and mononuclear cells Marta Ruiz-Ortega, Oscar Lorenzo, Jesus Egido

Typing of Clostridium difficile

A thiol antioxidant regulates IgE isotype switching by inhibiting activation of nuclear factor-κB Yukiyoshi Yanagihara, PhD, Yuji Basaki, MSc, Keiichi.

Impact of antibiotic restrictions: the patient's perspective

Comparative study of pediculicidal effect of medical plants

CMI readers' survey Clinical Microbiology and Infection

The future of diagnostic bacteriology

Analysis of TNF-α gene transcription and mRNA accumulation in THP-1 cells. Analysis of TNF-α gene transcription and mRNA accumulation in THP-1 cells. (A)

Presentation transcript:

Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level F. Araujo, T. Slifer, S. Li, A. Kuver, L. Fong, J. Remington Clinical Microbiology and Infection Volume 10, Issue 3, Pages 213-219 (March 2004) DOI: 10.1111/j.1198-743X.2004.00824.x Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Effect of different concentrations of gemifloxacin (Gemi) on secretion of IL-1α, IL-1,β IL-6, IL-10 and TNF-α by human monocytes (M) stimulated with lipopolysaccharide (LPS). + = p < 0.001 calculated for M only and M + LPS, while * = p < 0.01 calculated for M + LPS and M + LPS + concentration of Gemi. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Results of the electrophoretic shift-mobility assay. Lane 13 shows that cells incubated with gemifloxacin alone did not induce NF-ΚB activation. The locations of the unbound labelled oligonucleotide probe and the NF-ΚB-DNA complex are shown. Gemifloxacin concentrations of 0.001, 0.005 and 0.010 mg/L gave identical results. The results are representative of four separate experiments. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Effect of two different concentrations of gemifloxacin (Gemi) on secretion of TNF-α and IL-1β by LPS-stimulated THP-1 cells. Controls were THP-1 cells stimulated with LPS only. * = p < 0.01. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 Northern blot analysis of the effect of different concentrations of gemifloxacin (Gemi) on the expression of TNF-α mRNA in LPS-stimulated THP-1 cells. Controls were THP-1 cells stimulated with LPS only. The mRNA readings were normalised to human β-actin and the signals are expressed as absorbency units. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions