Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level  F. Araujo, T. Slifer, S.

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Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level  F. Araujo, T. Slifer, S. Li, A. Kuver, L. Fong, J. Remington  Clinical Microbiology and Infection  Volume 10, Issue 3, Pages 213-219 (March 2004) DOI: 10.1111/j.1198-743X.2004.00824.x Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Effect of different concentrations of gemifloxacin (Gemi) on secretion of IL-1α, IL-1,β IL-6, IL-10 and TNF-α by human monocytes (M) stimulated with lipopolysaccharide (LPS). + = p < 0.001 calculated for M only and M + LPS, while * = p < 0.01 calculated for M + LPS and M + LPS + concentration of Gemi. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Results of the electrophoretic shift-mobility assay. Lane 13 shows that cells incubated with gemifloxacin alone did not induce NF-ΚB activation. The locations of the unbound labelled oligonucleotide probe and the NF-ΚB-DNA complex are shown. Gemifloxacin concentrations of 0.001, 0.005 and 0.010 mg/L gave identical results. The results are representative of four separate experiments. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Effect of two different concentrations of gemifloxacin (Gemi) on secretion of TNF-α and IL-1β by LPS-stimulated THP-1 cells. Controls were THP-1 cells stimulated with LPS only. * = p < 0.01. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 4 Northern blot analysis of the effect of different concentrations of gemifloxacin (Gemi) on the expression of TNF-α mRNA in LPS-stimulated THP-1 cells. Controls were THP-1 cells stimulated with LPS only. The mRNA readings were normalised to human β-actin and the signals are expressed as absorbency units. Clinical Microbiology and Infection 2004 10, 213-219DOI: (10.1111/j.1198-743X.2004.00824.x) Copyright © 2004 European Society of Clinical Infectious Diseases Terms and Conditions