Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy

Slides:

Advertisements

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Advertisements

Nine Novel Germline Gene Variants in the RET Proto-Oncogene Identified in Twelve Unrelated Cases Syed A. Ahmed, Karen Snow-Bailey, W. Edward Highsmith,

CC Chemokine Receptor 5 and CXC Chemokine Receptor 6 Expression by Lung CD8+ Cells Correlates with Chronic Obstructive Pulmonary Disease Severity Christine.

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Volume 62, Issue 6, Pages (December 2002)

Kazunori Kanehira, Douglas L. Riegert-Johnson, Dong Chen, Lawrence E

A Genome-Wide High-Resolution Array-CGH Analysis of Cutaneous Melanoma and Comparison of Array-CGH to FISH in Diagnostic Evaluation Lu Wang, Mamta Rao,

Automated Quantitative RNA in Situ Hybridization for Resolution of Equivocal and Heterogeneous ERBB2 (HER2) Status in Invasive Breast Carcinoma Zhen.

Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer Qi-Peng Sun, Liao-Yuan.

Droplet Digital PCR for Absolute Quantification of EML4-ALK Gene Rearrangement in Lung Adenocarcinoma Qiushi Wang, Xin Yang, Yong He, Qiang Ma, Li Lin,

Tracy I. George, Joanna E. Wrede, Charles D. Bangs, Athena M

Detection of TMPRSS2-ETS Fusions by a Multiprobe Fluorescence in Situ Hybridization Assay for the Early Diagnosis of Prostate Cancer Qi-Peng Sun, Liao-Yuan.

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses Narasimhan Nagan, Nicole E. Faulkner,

Maria J. Worsham, Sandra R. Wolman, Richard J. Zarbo

Automated Quantitative RNA in Situ Hybridization for Resolution of Equivocal and Heterogeneous ERBB2 (HER2) Status in Invasive Breast Carcinoma Zhen.

Joanna Wang, Chetan Bettegowda The Journal of Molecular Diagnostics

Comparison of High-Resolution Melting Analysis, TaqMan Allelic Discrimination Assay, and Sanger Sequencing for Clopidogrel Efficacy Genotyping in Routine.

Sarah Chiang, Ladan Fazlollahi, Anhthu Nguyen, Rebecca A

Kazunori Kanehira, Douglas L. Riegert-Johnson, Dong Chen, Lawrence E

CRISPR/Cas9 Technology–Based Xenograft Tumors as Candidate Reference Materials for Multiple EML4-ALK Rearrangements Testing Rongxue Peng, Rui Zhang,

Fluorescence in Situ Hybridization Analysis of Immunoglobulin Heavy Chain Translocations in Plasma Cell Myeloma Using Intact Paraffin Sections and Simultaneous.

Molecular Diagnosis in Ewing Family Tumors

A Lean Laboratory The Journal of Molecular Diagnostics

Mucinous Differentiation Correlates with Absence of EGFR Mutation and Presence of KRAS Mutation in Lung Adenocarcinomas with Bronchioloalveolar Features

ALK Status Testing in Non–Small Cell Lung Carcinoma

The Development of a Multitarget, Multicolor Fluorescence in Situ Hybridization Assay for the Detection of Urothelial Carcinoma in Urine Irina A. Sokolova,

BK virus colonic ulcerations

Detection of TERC Amplification in Cervical Epithelial Cells for the Diagnosis of High- Grade Cervical Lesions and Invasive Cancer Jing Jiang, Li-Hui.

Getting Things Backwards to Prevent Primer Dimers

Infantile nephropathic cystinosis

Patrick R. Murray The Journal of Molecular Diagnostics

William L. Gerald, M.D., Ph.D, 1954–2008

Sean C. Murphy, Daniel R. Hoogestraat, Dhruba J

Identification of Combinatorial Genomic Abnormalities Associated with Prostate Cancer Early Recurrence Xiaoyu Qu, Claudio Jeldres, Lena Glaskova, Cynthia.

A Genome-Wide High-Resolution Array-CGH Analysis of Cutaneous Melanoma and Comparison of Array-CGH to FISH in Diagnostic Evaluation Lu Wang, Mamta Rao,

Pedram Gerami, Susan S. Jewell, Pedram Pouryazdanparast, Jeffery D

The Human Androgen Receptor X-Chromosome Inactivation Assay for Clonality Diagnostics of Natural Killer Cell Proliferations Michaël Boudewijns, Jacques.

Development of an NPM1/MLF1 D-FISH Probe Set for the Detection of t(3;5)(q25;q35) Identified in Patients with Acute Myeloid Leukemia Umut Aypar, Ryan.

Quiz Page January 2016 American Journal of Kidney Diseases

Chromosomal Biomarkers for Detection of Human Papillomavirus Associated Genomic Instability in Epithelial Cells of Cervical Cytology Specimens Irina.

BK Virus Infection Is Associated with Hematuria and Renal Impairment in Recipients of Allogeneic Hematopoetic Stem Cell Transplants Peter H. O'Donnell,

Next-Generation Sequencing for Simultaneous Determination of Human Papillomavirus Load, Subtype, and Associated Genomic Copy Number Changes in Tumors

Raymond R. Tubbs, James Pettay, Pat Roche, Mark H

BRAF Mutation Testing in Solid Tumors

Skin Biopsy Is a Practical Approach for the Clinical Diagnosis and Molecular Genetic Analysis of X-Linked Alport's Syndrome Fang Wang, Dan Zhao, Jie.

The MECT1-MAML2 Gene Fusion and Benign Warthin's Tumor

Volume 84, Issue 2, Pages (August 2013)

A Practical Approach to the Detection of Prognostically Significant Genomic Aberrations in Multiple Myeloma Zhong Chen, Bonnie Issa, Shiang Huang, Emily.

The Molecular Pathology of Primary Immunodeficiencies

Quiz Page December 2008 American Journal of Kidney Diseases

Polyoma Virus Infection

Molecular Cytogenetic Analyses of Immunoglobulin Loci in Nodular Lymphocyte Predominant Hodgkin's Lymphoma Reveal a Recurrent IGH-BCL6 Juxtaposition

Successful Application of a Direct Detection Slide-Based Sequential Phenotype/Genotype Assay Using Archived Bone Marrow Smears and Paraffin Embedded.

Array Comparative Genomic Hybridization Detects Chromosomal Abnormalities in Hematological Cancers That Are Not Detected by Conventional Cytogenetics

Retrospective Comparison of Nucleic Acid Sequence–Based Amplification, Real-Time PCR, and Galactomannan Test for Diagnosis of Invasive Aspergillosis

Automated Multiplexing Quantum Dots in Situ Hybridization Assay for Simultaneous Detection of ERG and PTEN Gene Status in Prostate Cancer Wenjun Zhang,

Molecular Monitoring of Chronic Myeloid Leukemia

The Detection of t(14;18) in Archival Lymph Nodes

Long Polymerase Chain Reaction-Based Fluorescence in Situ Hybridization Analysis of Female Carriers of X-Linked Chronic Granulomatous Disease Deletions

Ayalew Tefferi, Pierre Noel, Curtis A. Hanson

Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy

Karen Snow-Bailey, Ph.D., 1961–2006

Jie Hu, Malini Sathanoori, Sally J. Kochmar, Urvashi Surti

Angiotensin I-Converting Enzyme

Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards

Diagnostic Impact of Fluorescence in Situ Hybridization in the Differentiation of Hepatocellular Adenoma and Well-Differentiated Hepatocellular Carcinoma

The History and Impact of Molecular Coding Changes on Coverage and Reimbursement of Molecular Diagnostic Tests Susan J. Hsiao, Mahesh M. Mansukhani,

Polyoma Virus Infection

Presentation transcript:

Diagnosis of BK Viral Nephropathy in the Renal Allograft Biopsy Zhen Wang, Bryce P. Portier, Bo Hu, Andres Chiesa-Vottero, Jonathan Myles, Gary W. Procop, Raymond R. Tubbs The Journal of Molecular Diagnostics Volume 14, Issue 5, Pages 494-500 (September 2012) DOI: 10.1016/j.jmoldx.2012.04.004 Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Representative photomicrographs of a renal allograft biopsy, showing nuclear inclusions in tubular epithelial cell nuclei consistent with BK virus cytopathic effect; H&E (A) and PAS (B). The Journal of Molecular Diagnostics 2012 14, 494-500DOI: (10.1016/j.jmoldx.2012.04.004) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 IHC results for SV40 are displayed. Representative image of negative control (A) and representative image of a case positive for BK nephropathy (B). Original magnification: ×20. The Journal of Molecular Diagnostics 2012 14, 494-500DOI: (10.1016/j.jmoldx.2012.04.004) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Representative FISH image of a renal allograft biopsy. Individual green signals for chromosome 8 confirm the presence of successful hybridization in both BK positive (red) and BK negative (blue) nuclei. Original magnification, ×60. The Journal of Molecular Diagnostics 2012 14, 494-500DOI: (10.1016/j.jmoldx.2012.04.004) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 4 CISH results for BK virus (red) and IHC for CD3 (brown) CISH-PRO images. BK virus and CD3 detection via CISH-PRO in the absence (A) and in the presence (B) of significant interstitial or intratubular inflammation. The Journal of Molecular Diagnostics 2012 14, 494-500DOI: (10.1016/j.jmoldx.2012.04.004) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions