Transbronchial gene transfer of endothelial nitric oxide synthase to transplanted lungs Anders Jeppsson, MD, Carlo Pellegrini, MD, Timothy O’Brien, MD,

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Transbronchial gene transfer of endothelial nitric oxide synthase to transplanted lungs Anders Jeppsson, MD, Carlo Pellegrini, MD, Timothy O’Brien, MD, PhD, Virginia M. Miller, PhD, Henry D. Tazelaar, MD, Christopher G.A. McGregor The Annals of Thoracic Surgery Volume 66, Issue 2, Pages 318-323 (August 1998) DOI: 10.1016/S0003-4975(98)00552-9

Fig 1 Polymerase chain reaction of DNA extracted from rat lungs. A 356-bp band for ecNOS DNA is seen only in ecNOS-transduced lungs. (β-Gal = β-galactosidase.) The Annals of Thoracic Surgery 1998 66, 318-323DOI: (10.1016/S0003-4975(98)00552-9)

Fig 2 Reverse transcriptase polymerase chain reaction of messenger RNA extracted from rat lungs. A 250-bp band for ecNOS messenger RNA is seen only in ecNOS-transduced lungs. Messenger RNA for G3PDH was detected in all samples (450 bp). The Annals of Thoracic Surgery 1998 66, 318-323DOI: (10.1016/S0003-4975(98)00552-9)

Fig 3 Immunostaining for ecNOS in β-galactosidase (β-Gal)-transduced, transplanted lungs (A) and ecNOS-transduced, transplanted lungs (B). Arrows denote positive staining. Airway epithelium and vascular endothelial cells stained positively for eNOS in lungs from β-galactosidase–transduced lungs (arrows). Pneumocytes showed positive staining for ecNOS only in ecNOS-transduced lungs (arrow). (×200 before 50% reduction.) The Annals of Thoracic Surgery 1998 66, 318-323DOI: (10.1016/S0003-4975(98)00552-9)

Fig 4 Calcium-dependent (Ca Dep; black bars) and calcium-independent (Ca Indep; white bars) nitric oxide synthase activity in nontransfected lungs (CONTROLS) and lungs transfected with β-galactosidase (β-GAL) or endothelial nitric oxide synthase (ecNOS). Data are shown as mean ± standard error of the mean, n = 4 in each group. Asterisk denotes statistical significance from control (analysis of variance, p < 0.05). The Annals of Thoracic Surgery 1998 66, 318-323DOI: (10.1016/S0003-4975(98)00552-9)