Sensitivity and specificity of TCSn::LUC in transient transfection assays. Sensitivity and specificity of TCSn::LUC in transient transfection assays. A,

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Sensitivity and specificity of TCSn::LUC in transient transfection assays. Sensitivity and specificity of TCSn::LUC in transient transfection assays. A, Induction of ARR6::LUC, TCS::LUC, and TCSn::LUC to increasing concentrations of transzeatin. B, TCSn::LUC is induced by transzeatin, but not by auxin, GA3, or abscisic acid. AtGH3::LUC and RD29A::LUC serve as positive controls for auxin and abscisic acid hormone induction, respectively (Müller and Sheen, 2008). C, Cytokinin-dependent induction of TCSn::LUC is compromised in ahk4 ahk3, ahk4 ahk2, and ahk2 ahk3 double mutant cells. cre1-12 is a mutant allele of AHK4 (Higuchi et al., 2004). D, Positive regulators of the MSP network induce TCSn::LUC expression. APRR2 and LUX have no effect. E, Type-A and type-C ARR attenuate cytokinin-dependent induction of TCSn::LUC. F, TCS::LUC and TCSn::LUC are induced in maize protoplasts by transzeatin. tz, Transzeatin; IAA, auxin; GA, GA3; ABA, abscisic acid. Evelyne Zürcher et al. Plant Physiol. 2013;161:1066-1075 ©2013 by American Society of Plant Biologists