SpA-SEA-D227A-induced CTL lyse MAb-coated EC

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SpA-SEA-D227A-induced CTL lyse MAb-coated EC SpA-SEA-D227A-induced CTL lyse MAb-coated EC. (A) A strong SADCC was induced by SpA-SEA-D227A targeted to HLA class I-expressing EA.hy926 cells that had been preactivated in the presence of TNF-α (▵) compared to unstimulated controls (■). SpA-SEA-D227A-induced CTL lyse MAb-coated EC. (A) A strong SADCC was induced by SpA-SEA-D227A targeted to HLA class I-expressing EA.hy926 cells that had been preactivated in the presence of TNF-α (▵) compared to unstimulated controls (■). (B) E:T ratios are shown for SADCC induced by SpA-SEA-D227A (1.0 ng/ml) by using anti-HLA class I-coated target cells with (▵) and without (■) TNF-α preactivation. (C) Cytotoxicity was induced when SpA-SEA-D227A was targeted to the cell surface by using anti-CD54 MAb. TNF-α-activated EA.hy926 cells (⧫ and ▵) were compared to unstimulated cells (■ and ○). The results of two separate experiments are indicated. Experimental procedures were as indicated in the legend to Fig. 2. In panels A and B, the results of one out of three representative experiments are shown. Kristian Riesbeck et al. Clin. Diagn. Lab. Immunol. 1998; doi: