Structural and Thermodynamic Principles of Viral Packaging

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Date of download: 7/10/2016 Copyright © ASME. All rights reserved. From: A Model for Highly Strained DNA Compressed Inside a Protein Cavity J. Comput.
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Structural and Thermodynamic Principles of Viral Packaging Anton S. Petrov, Stephen C. Harvey  Structure  Volume 15, Issue 1, Pages 21-27 (January 2007) DOI: 10.1016/j.str.2006.11.013 Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 1 Coarse-Grained Representation of Bacteriophage ϕ29 (A) Model capsid for ϕ29, constructed by elongating a pentakisdodecahedron and inserting 20 additional faces plus a cylinderical core that is 146 Å long and 108 Å in diameter (Tao et al., 1998). (B) Space-filling model for the ϕ29 capsid; each triangular face of the polyhedron was filled with pseudoatoms with radii of 8 Å. (C) A typical conformation resulting from a 70 μs MD simulation of packing DNA inside ϕ29. Individual pseudoatoms (six base pairs) are shown in this representation, clearly illustrating the folded toroidal geometry. A rainbow coloring scheme was used to distinguish between the head (red) and tail (blue) of the DNA. Note that the capsid and connector are not shown. Other conformations are shown in Figure S1. Structure 2007 15, 21-27DOI: (10.1016/j.str.2006.11.013) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 2 Thermodynamic Quantities Calculated upon DNA Injection into Bacteriophage ϕ29 (A) The force versus the percentage of ϕ29 genome packed. (B) The Helmholtz free energy change, ΔA, obtained by integrating the force versus distance curves; the internal free energy change, ΔU, calculated as the sum of the force-field terms (packaged DNA − free DNA), and the entropic term, obtained by subtraction, −TΔS = ΔA − ΔU. (C) Decomposition of the total internal energy change, ΔU, into its stretching, bending, electrostatic, and “soft sphere” volume-exclusion components. All thermodynamic quantities were calculated for each of ten individual conformations and averaged. Error bars represent standard deviations. Structure 2007 15, 21-27DOI: (10.1016/j.str.2006.11.013) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 3 Reconstructed Density Maps for DNA Packaged inside the ϕ29 Capsid (A–C) The DNA density is shown in red; capsid and core densities are shown in blue and yellow, respectively. (A) Equatorial cross-section. (B) Longitudinal cross-section. (C) A cross-sectional view to show the back half of the structure. Note that the concentric organization in the density map averaged over multiple conformations is identical to those seen experimentally (Jiang et al., 2006; Tao et al., 1998), but one could not infer the individual folded toroidal conformation seen in Figure 1C from the density reconstruction. Structure 2007 15, 21-27DOI: (10.1016/j.str.2006.11.013) Copyright © 2007 Elsevier Ltd Terms and Conditions