M. Schwaiger, O. Péter, P. Cassinotti

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Routine diagnosis of Borrelia burgdorferi (sensu lato) infections using a real-time PCR assay M. Schwaiger, O. Péter, P. Cassinotti Clinical Microbiology and Infection Volume 7, Issue 9, Pages 461-469 (September 2001) DOI: 10.1046/j.1198-743x.2001.00282.x Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Multiple alignment of seven published flagellin gene sequences of Borrelia burgdorferi (sensu lato) strains. The nucleotides identical among all strains are indicated with asterisks in the consensus sequence. The forward primer FlaF1A, the reverse primer FlaR1 and the TaqMan probe FlaProbe 1 are printed in bold. The accession numbers (NCBI) of the flagellin gene sequences used for the comparison are: D63365(Borrelia afzellii VS461), X75202(Borrelia afzelii ACAI), L42885(Borrelia burgdorferi IP90), D63367(Borrelia garinii HT22), D63366(Borrelia afzelii HT61), X15660(Borrelia burgdorferi GeHo), and X15661 (Borrelia burgdorferi B31). Clinical Microbiology and Infection 2001 7, 461-469DOI: (10.1046/j.1198-743x.2001.00282.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Amplification plot (a) and standard curve (b) of the Borrelia burgdorferi quantitative assay. Clinical Microbiology and Infection 2001 7, 461-469DOI: (10.1046/j.1198-743x.2001.00282.x) Copyright © 2001 European Society of Clinical Infectious Diseases Terms and Conditions